TY - JOUR AU - Salvioni Recalde, Oscar Daniel AU - Fraenkel, Stefanía AU - José Tintel, María AU - Arze, Verónica Paola AU - Ramírez Centurión, Natalia AU - Rolón, Miriam AU - Vega Gómez, Celeste PY - 2021/04/06 Y2 - 2024/03/28 TI - First report of the presence of Trypanosoma evansi in dogs from Paraguay applying molecular techniques JF - Brazilian Journal of Veterinary Medicine JA - Braz. J. Vet. Med. VL - 43 IS - 1 SE - Case report DO - 10.29374/2527-2179.bjvm001920 UR - https://bjvm.org.br/BJVM/article/view/1171 SP - e001920 AB - <p>Surra is a disease caused by the hemoflagellate pathogen Trypanosoma evansi which affects a wide variety of mammals. The only cases reported of the presence of T. evansi in Paraguay were identified in samples from horses and capybaras and they were detected by light microscopy in the 19th century. The aim of this study is to report three autochthonous cases of canine trypanosomiasis caused by T. evansi, for the first time in the country, using molecular techniques and their application for the differential diagnosis of trypanosomatids species. The technique implemented was real-time PCR-HRM, amplifying a fragment of the hsp70&nbsp;gene, using a pair of primers initially used to discriminate Leishmania species. This is the first report on the usage of these primers to detect T. evansi through HRM analysis, which allows the&nbsp;differentiation of trypanosomatids species simultaneously, making it an efficient tool for differential diagnosis.</p> ER -