@article{da Silva Soares_Couto da Motta_Lima Barbieri_Araújo de Melo_Alvim Gomez_Abreu de Alencar_da Silva Coelho_de Mattos de Oliveira Coelho_Mary Logue_Moreira Soares de Souza_2021, title={ Molecular characterization and genetic diversity of Staphylococcus aureus isolates of dairy production farms in Rio de Janeiro, Brazil}, volume={43}, url={https://bjvm.org.br/BJVM/article/view/1173}, DOI={10.29374/2527-2179.bjvm001120}, abstractNote={<p><em>Staphylococcus aureus </em>is an important pathogen involved in subclinical bovine mastitis, causing high economic losses for the dairy industry. The successful persistence of this pathogen in the host occurs due to a series of factors associated with colonization ability and the acquisition of virulence factors. This bacterial species carries genetic heterogeneity, and genetically diverse strains characterize the population. Analysis of the genetic variation is an important tool for epidemiological studies. For this study, <em>S. aureus </em>strains were randomly selected by molecular profiling. All strains were originated from the milk of cows of subclinical mastitis on farms in the State of Rio de Janeiro. Strains of <em>S. aureus </em>were profiled using virulence gene analysis profiles, <em>agr </em>and <em>spa </em>typing, Pulsed Field Gel Electrophoresis (PFGE), and Multilocus Sequence Typing (MLST). 47% (8/17) of the tested strains were positive strains for <em>ica</em>A gene; 82.3% (14/17) for <em>ica</em>D gene; 41% (7/17) for <em>fbn</em>A gene; 47% (8/17) positive for <em>fbn</em>B gene; 94% (16/17) for the <em>hl</em>A gene and 70.5% (12/17) for <em>hl</em>B gene. These virulence results generated 11 different profiles. Most strains (58.8% - 10/17) were classified as type-II by <em>agr </em>system. <em>spa </em>typing identified seven different spa types. PFGE analysis found extensive genetic heterogeneity and no clones were observed. MLST analysis generated five different types of ST/CC. Considering the results observed at the present study, a high genetic variety of <em>S. aureus </em>strains associated of the presence of different virulence factors justified the absence of clonal strains at the properties evaluated. Besides, that, the definition of <em>S. aureus </em>clonal strain as well dynamic population in not fully understood since there are a limited number of studies in <em>S. aureus </em>associated with bovine mastitis.</p>}, number={1}, journal={Brazilian Journal of Veterinary Medicine}, author={da Silva Soares, Bianca and Couto da Motta, Cássia and Lima Barbieri, Nicolle and Araújo de Melo, Dayanne and Alvim Gomez, Marisol and Abreu de Alencar, Tatiani and da Silva Coelho, Irene and de Mattos de Oliveira Coelho, Shana and Mary Logue, Catherine and Moreira Soares de Souza, Miliane}, year={2021}, month={Apr.}, pages={e001120} }