Control of experimental liver injury in mice left medial lobe

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Gabriel Barbosa Botelho de Souza
Edmundo Jorge Abílio
Priscilla Olivieri Benck de Jesus
Alexandra de Faria do Amaral
Fabiane Azeredo Atallah
Saulo José Quina Silva
Guilherme Alexandre Soares Monteiro
Fernanda Antunes


ABSTRACT. Souza G.B.B., Abílio E.J., Jesus P.O.B., Amaral A.F., Atallah F.A., Silva S.J.Q., Monteiro G.A.S. & Antunes F. [Control of experimental liver injury in mice left medial lobe]. Controle de dano experimental em lobo medial esquerdo hepático de ratos. Revista Brasileira de Medicina Veterinária, 35(Supl. 1):30-44, 2013. Curso de Pós-Graduação em Ciência Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ 28013602, Brasil. Email: The surgery of damage control consists of maneuver that can save lives, consisting of stop the bleeding, control the output of intestinal contents, among other, avoiding the resections and reconstructions. The challenge based on maintenance of physiological stability after injury by surgical control of the hemorrhage. Even with all the efforts, the disease evolves with a cascade of events that includes the so-called triad of death: metabolic acidosis, hypothermia, and coagulopathy. Thus, the aim of this study was to evaluate the damage control in injuries caused experimentally on hepatic lobe of Wistar rats, proposing four alternatives for damage control, confronting with histopathological data of the site of the lesion after repair. A total of 24 Wistar rats of both sexes, weight ranging between 250 and 300g were disposed in four randomized blocks, all anesthetized with a combination of ketamine and xylazine by the intraperitoneal route. After anesthesia the experimental lesion was conducted by thelaser of scalpel into hepatic lobe which is the closest to the area of the incision chosen. Later, animals were separated in groups and treatments consisting in group 1, using as a method to manual compression of the injured region (digitocrasia); Group 2, where the passage of omentum through the lesion; Group 3, with the placing of compresses folded around the injury and the Group 4, in which the fulfillment of the liver with bovine pericardium glycerin distilled preserved. After lesion cavity suture was performed and the animals evaluated daily and after seven days were re-operated for the withdrawal of the repair fragment procedure and histopathological analysis in the Laboratory of Morphology and Animal Pathology (LMPA) of Veterinary Hospital UENF. The fragment was categorized macroscopically by shape, texture, consistency and color of the surfaces. The portion removed was immediately fixed in formalin neutral-buffered 10% for a minimum period of 48 hours and after the whole process of histotecnica. The tissue regeneration was scored by descriptive statistics. During the surgical procedure the data showed that all the proposed techniques were effective in hemostasis, given that the bleeding was constrained in all the groups, especially in groups 1 and 2 that was observed an immediate hemostasis, when compared with other two groups. At the time of collection of the fragment of repair, was observed discrepancy among the groups. Macroscopic observation showed that the region repaired presented significant differences, however in groups using packing the prevalence of adherence phenomena was higher than the other groups. Besides all adherence observed, group 4 presented a further discoloration, with a whitish color, in the area of hepatic lobe involved by preserved bovine pericardium. Microscopically the differences were even greater among the groups, with the extent of the lesions ranging from absent to intense, revealing degeneration and liver cirrhosis in all segments of the study. However in group 4 the severity observed was higher and comparatively more deleterious extending to adjacent lobe which also presented dysfunction based on degeneration and congestion.

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de Souza, G. B. B., Abílio, E. J., de Jesus, P. O. B., do Amaral, A. de F., Atallah, F. A., Silva, S. J. Q., Monteiro, G. A. S., & Antunes, F. (2012). Control of experimental liver injury in mice left medial lobe. Brazilian Journal of Veterinary Medicine, 35(Supl.1), 30–44. Retrieved from
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