ABSTRACT. Leal P.D.S.A., Campos D.P., Rodrigues M. deL. deA., Botelho, G.G., Labarthe N.V. & Lopes C.W.G. [Gastrointestinal parasites in a colony of cats in the West Zone of the city of Rio de Janeiro, Brazil.] Parasitos gastrintestinais em uma colônia de gatos na Zona Oeste da cidade do Rio de Janeiro, Brasil. Revista Brasileira de Medicina Veterinária, 37(Supl.1):95-99, 2015. Curso de Pós-Graduação em Ciências Veterinárias, Anexo 1, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Campus Seropédica, Seropédica, RJ 23897-970, Brasil. E-mail: email@example.com The study of parasitic diseases of domestic cats is of great importance to public health because some helminthes and coccidia are considered zoonotic. This survey aimed to mark the gastrointestinal parasites in a shelter for cats in the West Zone of the City of Rio de Janeiro, RJ. To this end, stool samples were collected from 16 cats born and living in this colony. Two techniques were used in the diagnosis of centrifugal flotation in saturated sucrose solution (CFSSS) and sedimentation by centrifugation in formalin-ether (SCFE). The forms of the parasites found were grouped according to the morphological characteristics of their eggs in roundworms, hookworms, whipworm and Platynosomum illiciens, and sporulated oocysts as Cystoisospora rivolta and C. felis. The stool tests were performed by using two techniques foregoing and they were not significant, except for P. illiciens eggs where the SCFE (p = 0.0290) was more effective than CFSSS in the identification of feline liver fluke infection. In spite of hookworm infection had been more prevalent with 87.5% of infected animals, feline liver fluke due to P. illiciens was 43.75%(7/16) of the infected animals. As for the multiple infections, the most frequent was the association of hookworm + C. felis + C. rivolta with 31.25% (5/16) of the infected animals, followed by C. felis + C. rivolta + Ancilostomatídeos + Ascarídeos with 18.75% (3/16). The exception of an animal which had all gastrointestinal parasites observed in stools of sheltered cats. The combination of the two techniques is an important tool in detecting eggs, when there is suspicion of P. illiciens infection in the cat population. In other infections, even in the multiple infections, both techniques could be used in the identification of helminth eggs and coccidia oocysts as observed in the present study.