Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses
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Keywords

autohemoterapia, cavalo, citocinas, fagocitose. autohemotherapy, horse, cytokines, phagocytosis.

How to Cite

Lopes, P. R., Batista, M. A., Soares, R. P., de Oliveira, . J. G., Leme, F. de O. P., Martins-Filho, O. A., Maranhão, R. de P. A., Wenceslau, R. R., Palhares, M. S., & Araújo, M. S. S. (2021). Autohemotherapy increases phagocytic activity of neutrophils and promotes cytokine production by lymphocytes in horses. Brazilian Journal of Veterinary Medicine, 43(1), e000821. https://doi.org/10.29374/2527-2179.bjvm000821

Abstract

Autohemotherapy (AHT) is a procedure in which blood is collected by venipuncture, followed by immediate reinjection to activate an animal’s immune system. In this preliminary study, we evaluated the effect of AHT on clinical, hematological and biochemical parameters and immune responses in horses. Eight healthy animals (four males and four females) were clinically evaluated and subjected to AHT (three applications at a weekly interval). Blood samples were collected before AHT (T0) and 3 d after each procedure (T1, T2, and T3). AHT did not affect most clinical, biochemical, and hematological parameters in the test animals. However, flow cytometry detected an increase in T lymphocytes (CD3+) and cytokine production (IFN-γ, IL-10, IL-4, and IL-6). In addition, AHT stimulated phagocytosis by neutrophils at T3. Although preliminary, our results suggest that AHT is a promising procedure for equine management, as AHT was found to stimulate the horses’ immune responses without affecting their general status.

https://doi.org/10.29374/2527-2179.bjvm000821
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Copyright (c) 2021 Paula Rodrigues Lopes, Mauricio Azevedo Batista, Rodrigo Pedro Soares, Jéssica Guerra de Oliveira, Fabiola de Oliveira Paes Leme, Olindo Assis Martins-Filho, Renata de Pino Albuquerque Maranhão, Raphael Rocha Wenceslau, Maristela Silveira Palhares, Marcio Sobreira Silva Araújo