Brazilian Journal of Veterinary Medicine <pre id="tw-target-text" class="tw-data-text tw-ta tw-text-small" dir="ltr" data-placeholder="Tradução" data-fulltext=""><span lang="en"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">O Jornal Brasileiro de Medicina Veterinária (BJVM) é o jornal oficial da Sociedade de Medicina Veterinária do Rio de Janeiro (Somverj). </span></span><br></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Como uma política editorial, publica artigos científicos originais, revisões (sem convite do Editor) e relatos de casos. </span></span><br></span><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Em todos os campos da Medicina Veterinária. </span></span></span></span><br></span></pre> Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. en-US Brazilian Journal of Veterinary Medicine 0100-2430 Complex Leopard non-associated congenital night blindness in Mangalarga Marchador foals <p>The report describes two cases of four- and five-month-old Mangalarga Marchador fillies showing signs of night blindness. The animals appeared to have disorientation at night. Ophthalmic examination revealed all parameters as normal except for the absence of menace response in the penumbra. Fundoscopy examination did not demonstrate any morphological abnormalities in the retina. Electroretinography examination confirmed a failure in the electrical activity of rods, which are retinal photoreceptors responsible for night vision. However, we noted a small reduction in the electrical activity of the cones, suggesting that the animals may have visual difficulties in clear environments. Genomic analysis of the hair follicles from their mane (Veterinary Genetics Laboratory/UC – Davis, USA) did not reveal the presence of homozygosity for the Leopard Complex, a gene pool associated with congenital stationary nocturnal blindness (CSNB) in horses with depigmented hair. Outcomes of the studbook analysis suggested the presence of kinship among the described animals. Additional research is required for the genomic determination of nocturnal blindness in Mangalarga Marchador horses.&nbsp;</p> Daniele Mancilha Carneiro Camilla Espíndula Mendonça Maurílio Rosa Denise Monnerat Nogueira Leonardo Rodrigues de Lima Copyright (c) 2020 Daniele Mancilha Carneiro, Camilla Espíndula Mendonça, Maurílio Rosa, Denise Monnerat Nogueira, Leonardo Rodrigues de Lima 2020-04-18 2020-04-18 42 1 e106220 e106220 10.29374/2179.bjvm106220 ACQUIRED MYASTHENIA GRAVIS IN DOG – CASE REPORT <p>Neuromuscular diseases are uncommon in the clinical routine of dogs and cats and their diagnosis is a challenge for the veterinarian. Thus, this paper reports the clinical diagnosis and established therapy in a four-year-old female without a defined breed with a history of ataxia and tetraparesis, especially after exercise. Due to the history and clinical signs presented, acquired myasthenia gravis was suspected and the therapeutic diagnosis was chosen using anticholinesterase neostigmine, for being simpler, faster and more accessible. The patient presented a significant improvement in clinical status after administration of neostigmine intramuscularly, returning to normal ambulatory activity after administration of the drug intravenously, thus making possible the diagnosis of the disease. Treatment was instituted using pyridostigmine bromide associated with corticosteroid in a immunosuppressive dose for a period of six months, observing complete remission of the clinical signs without recurrences until the present moment.</p> Maria Eduarda dos Santos Lopes Fernandes Gabriela Wacheleski Brock Anna Julia Rodrigues Peixoto Clarice Gonring Corrêa Patricia de Oliveira Alex Gradowski Adeodato Marta Fernanda Albuquerque da Silva Cássia Maria Molinaro Coelho Copyright (c) 2020 Maria Eduarda dos Santos Lopes Fernandes, Gabriela Wacheleski Brock, Anna Julia Rodrigues Peixoto, Clarice Gonring Corrêa, Patricia de Oliveira, Alex Gradowski Adeodato, Marta Fernanda Albuquerque da Silva, Cássia Maria Molinaro Coelho 2020-05-05 2020-05-05 42 1 e106320 e106320 10.29374/2527-2179.bjvm106320 Renal nephroblastoma in adult dog <p>Nephroblastoma is a neoplasia originating from the poor differentiation of the metanephrogenic blastema, therefore neoplasia of embryonic origin. It is commonly reported in dogs less than two years old and is rare in adults. Clinical signs include hematuria, polyuria and palpable abdominal mass. Surgery is elective therapy. Chemotherapy depend on the stage of the disease. The purpose of this paper is to report a case of nephroblastoma in a mixed-breed, six-year-old male canine with a stage I nephroblastoma (according to human staging for Wilm's tumor). The patient performed clinically well and without changes in the staging exams (thorax radiographs). The initial complete blood count indicated only lymphopenia and biochemical profile without alterations. Abdominal ultrasound showed vascularized formation with no defined limits, cystic, with probable origin in left kidney, dislocating liver and spleen. The recommended treatment was total nephrectomy. Kidney size was about 25.0 cm in diameter, with irregular surface. Numerous cysts were present and were covered by a capsule with brownish-coloured liquid and without adhering to adjacent structures. The nephroblastoma diagnosis was obtained by histopathological exam. No adjuvant chemotherapy treatment was carried out. The patient presented a survival of 33 months.</p> Dayane Caicó Collares Araujo Michel Alves da Silva Cristiano Chaves Pessoa da Veiga Julio israel Fernandes Copyright (c) 2020 Dayane Caicó Collares Araujo, Michel Alves da Silva , Cristiano Chaves Pessoa da Veiga, Julio israel Fernandes 2020-06-16 2020-06-16 42 1 e107820 e107820 10.29374/2527-2179.bjvm107820 Sucessful of nitempyram in treatment occular myiasis in orbital cavity in dog – case report <p>The aim of this study was to report the efficacy of nitempiram against Cochiliomiyia hominivorax larvae in ocular myiasis in a naturally infested dog. A female Beagle dog with occular miyasis was attended. After care and clinical examination, was found involvement of the entire ocular and periocular region. The animal was treated with nitenpyram, with a dose of 4,7 mg/kg, orally, jointly with analgesic medication. The expulsion and fall of live and dead larvae were evaluated, which were quantified every 15 minutes in the first hour and thereafter at 2, 3, 4, 6, 18 hours after treatment and subsequent manual removal of the larvae. Overall effectiveness was calculated, the larval expulsion rate and the larvicidal effect of nitenpyram. In full there were 140 larvae in the lesion, from which 90 live larvae were expelled, 46 dead larvae and 4 dead larvae mechanically removed, presenting 100% overall effectiveness, with larval expulsion activity of 97.1% and larvicidal efficacy of 35.7%. Based on the results obtained in this case, it can be concluded that nitenpyram has a fast-acting and safe action under <em>C. hominivorax </em>larvae, proving be a pleasant alternative therapeutic alternative for the elimination of myiasis in orbital cavity of dogs.</p> Fernando Rocha Miranda Renan Bernardes Tavares Ciro Eugênio da Silva de Oliveira Emily Andressa Santos Lima Diefrey Ribeiro Campos Fabio Barbour Scott Julio Israel Fernandes Copyright (c) 2020 Fernando Rocha Miranda, Renan Bernardes Tavares, Ciro Eugênio da Silva de Oliveira, Emily Andressa Santos Lima , Diefrey Ribeiro Campos, Fabio Barbour Scott, Julio Israel Fernandes 2020-02-13 2020-02-13 42 1 e108920 e108920 10.29374/2527-2179.bjvm108920 Thoracomelia in sheep: a case report <p>In this case report, the occurrence of thoracomelia in a female sheep is reported. Thoracomelia is a<br>congenital malformation that can affect several animal species and is characterized by the presence of<br>a supernumerary limb in the thoracic region. Scientific studies regarding the etiology of changes of this<br>nature are limited in veterinary medicine, especially concerning sheep. So far, there is no consensus<br>regarding if the origin of this congenital malformation is genetic or environmental. Here, we provide the<br>historical and photographic record of thoracomelia in a female sheep, and relate the malformation to an<br>environmental cause: physical aggression suffered during embryonic development. The sheep underwent<br>a surgical procedure for removal of the accessory limb and subsequently remained in the breeding line,<br>giving birth to healthy descendants without thoracomelia.</p> Eder Sardinha Silva Vanessa Silva Mustafa Paula Aguiar Sá Rita de Cassia Campebell Copyright (c) 2020 Eder Sardinha Silva, Vanessa Silva Mustafa, Paula Aguiar Sá, Rita de Cassia Campebell 2020-06-16 2020-06-16 42 1 e108220 e108220 10.29374/2527-2179.bjvm108220 Influence of obesity on the clinical improvement of tracheal and bronchial collapse in dogs: a case report <p class="western" lang="en-US"><span style="font-family: Lucida Sans, sans-serif;"><span style="font-size: medium;">Tracheal collapse is the most common progressive affection of the anterior pathways in small animals. The most common clinical symptom is a “goose honk” cough in cases of tracheal collapse and inspiratory discomfort in cases of cervical collapse. The diagnosis is based on the history, physical examination, and imaging tests, notably chest radiography and endoscopic examination. Treatment can be clinical or surgical depending on the degree of collapse. Obesity currently affects several domestic animals and predisposes them to respiratory disorders. The overlapping adipose tissue presses on the muscles of the trachea, aggravating the collapse and influencing the lung compliance and expansion. The present report describes the clinical improvement in an obese canine with tracheal and bronchial collapse after clinical management and therapeutic ration.</span></span></p> Nathália Marques de Oliveira Lemos Mario Santos Filho Camila de Oliveira Ferreira Mendes Juliana Silva do Carmo Eveliny Oliveira Eleutério Cristiano Chaves Pessoa da Veiga Jonimar Pereira Paiva Copyright (c) 2020 Nathália Marques de Oliveira Lemos, Mário dos Santos Filho, Camila de Oliveira Ferreira Mendes, Juliana Silva do Carmo, Eveliny Oliveira Eleutério, Cristiano Chaves Pessoa da Veiga, Jonimar Pereira Paiva 2020-09-23 2020-09-23 42 1 e107620 e107620 10.29374/10.29374/2527-2179.bjvm107620 Purulent fibrous and necrotizing ileitis secondary to intussus associated with infection by Giardia intestinalis and Cystoisospora felis in cat - case report <p>Intussusception is the prolapse of an intestinal segment in the lumen of the adjacent intestinal loop.<br>Although usually idiopathic, one of the predisposing factors is intestinal parasitism. The aim of this report<br>is to describe the unusual case of ileo-ileal intussusception in a kitten co-parasitized by Cystoisospora<br>felis and G. intestinalis A three-month-old mixed breed cat was treated after suffering from intermittent<br>vomiting, prostration, progressive weight loss, abdominal discomfort, and diarrhea for two days. The<br>presence of numerous <em>Giardia intestinalis</em> cysts and <em>C. felis</em> oocysts were detected in feces samples. The<br>therapeutic protocol did not produce the expected result, and the patient evolved to hypovolemic shock<br>and perished. During emergency care, it was possible to detect a palpable cylindrical abdominal mass<br>in the caudal region of the abdomen. At necropsy, necrotizing purulent fibrinous ileitis secondary to<br>intussusception was observed. Intestinal hyper-peristalsis associated with intense parasitism by these<br>protozoa suggested the participation of these parasites in the genesis of the disease. Early diagnosis of<br>infection by these parasites is vital to improve patient life quality and the accuracy of intussusception<br>prognosis due to intestinal disorders caused by parasitism.</p> Jéssica Karoline de Oliveira Chaves Emily Andressa Santos Lima Cecília Lopes da Conceição Debora Azevedo Borges Diefrey Ribeiro Campos Fabio Barbour Scott Copyright (c) 2020 Jéssica Karoline de Oliveira Chaves, Emily Andressa Santos Lima, Cecília Lopes da Conceição, Debora Azevedo Borges, Diefrey Ribeiro Campos, Fabio Barbour Scott 2020-09-29 2020-09-29 42 1 e107320 e107320 10.29374/10.29374/2527-2179.bjvm107320 Myxomatous degeneration of the left atrioventricular valve in a true parrot (Amazona aestiva): a case report <p>Owning wild species as pets is becoming more and more common. Consequently, experience in caring for them has grown, resulting in the diagnosis of numerous diseases. Among these are cardiovascular disorders, especially in parrots. However, the lack of specificity or the absence of clinical signs of these diseases make it difficult to assess cardiovascular issues during the physical examination of birds. This leads to underdiagnosis of these conditions, with the majority being diagnosed post-mortem. This study reports the cardiovascular findings in post-mortem examinations of individuals of the species <em>Amazona aestiva</em> (true parrot) of Centro de Triagem de Animais Silvestres do Rio de Janeiro (CETAS-RJ) and is the first record of myxomatous degeneration of the left atrioventricular valve in this species.</p> Andressa Kagohara Mário dos Santos Filho José Philippe Matieli Marinho Daniel Marchesi Neves Luciano da Silva Alonso Jonimar Pereira Paiva Daniel de Almeida Balthazar Copyright (c) 2020 Andressa Kagohara , Mário dos Santos Filho, José Philippe Matieli Marinho, Daniel Marchesi Neves, Luciano da Silva Alonso, Jonimar Pereira Paiva , Daniel de Almeida Balthazar 2020-10-22 2020-10-22 42 1 e104720 e104720 10.29374/2527-2179.bjvm104720 Feline intraocular sarcoma: case report <p>Ophthalmic disorders in cats are increasingly common in veterinarians’ professional routine. Eye neoplasms are important diseases that can lead to discomfort, visual loss and death. Feline intraocular sarcoma is a malignant neoplasm that can be diagnosed in cats of any age. In this report, a 6-month-old female cat was treated at the Veterinary Hospital of Federal Rural University of Rio de Janeiro, with the left eyeball in the phthisisbulbi stage resulting from a traumatic event four months previously. Enucleation surgery for the affected eye was performed. The histopathological evaluation of the eyeball revealed early-stage intraocular sarcoma</p> Isabela Pessôa Barbieri Bastos Camila Valério Baruel Thais Fontes Braga Rayssa Dias Faleiro Renan Schiebel Medeiros Leonardo Rodrigues de Lima Copyright (c) 2020 Isabela Pessôa Barbieri Bastos, Camila Valério Baruel, Thais Fontes Braga, Rayssa Dias Faleiro, Leonardo Rodrigues de Lima 2020-11-28 2020-11-28 42 1 e106920 e106920 10.29374/2527-2179.bjvm106920 Sucessful treatment of feline demodicosis with orally sarolaner – Case Report <p><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Demodicose é uma dermatose parasitária considerada incomum no gato doméstico. </span><span style="vertical-align: inherit;">Este estudo tem como objetivo relatar o primeiro sucesso terapêutico do sarolaner por via oral no tratamento de </span></span><em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Demodex </span></span></em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">spp. </span><span style="vertical-align: inherit;">em um gato naturalmente infestado no Brasil. </span><span style="vertical-align: inherit;">Um gato adulto, positivo para o vírus da leucemia felina e em tratamento com glicocorticóides, apresentava sinais clínicos da pele como prurido intenso, hiperceratose, lignificação, fragilidade da pele e alopecia simétrica. </span><span style="vertical-align: inherit;">O diagnóstico foi confirmado em arranhões na pele, impressão em tiras de acetato e exame coproparasitológico. </span><span style="vertical-align: inherit;">Após o diagnóstico, o sarolaner foi administrado por via oral (4mg / kg). </span><span style="vertical-align: inherit;">A avaliação foi realizada com arranhões na pele 24, 48 horas e 7, 14, 21 e 28 dias após o tratamento. </span><span style="vertical-align: inherit;">Após 21 dias, nenhum ácaro foi observado e houve uma melhora na fragilidade da pele e na repilação parcial. </span><span style="vertical-align: inherit;">Com base nos resultados deste estudo, pode-se concluir que o sarolaner foi eficaz no tratamento da sarna demodécica felina.</span></span></p> Gabriela Pereira Salça de Almeida Diefrey Ribeiro Campos Fabio Barbour Scott Thais Ribeiro Correia Copyright (c) 2020 Gabriela Pereira Salça de Almeida, Diefrey Ribeiro Campos, Fabio Barbour Scott, Thais Ribeiro Correia 2020-12-07 2020-12-07 42 1 e113420 e113420 10.29374/2527-2179.bjvm113420 Prolapse of the third eyelid gland in a mixed breed cat: case report <p>A 4-year-old male mixed-breed cat was evaluated with a history of the presence of a reddish mass in the left eye medial corner. Ophthalmic examination revealed prolapse of the third eyelid gland, conjunctival secretion and hyperemia. The animal underwent gland replacement surgery using the modified Morgan technique. After surgery, clinical signs improved on the first day. The cat was observed for 2 years after surgery and there was no recurrence of the condition. The surgical technique was effective in resolving the condition.</p> Isabela Pessoa Barbieri Bastos Rayssa Dias Faleiro Gabriela Pereira Salça de Almeida Jessica Karoline de Oliveira Chaves Diefrey Ribeiro Campos Copyright (c) 2020 Isabela Pessoa Barbieri Bastos, Rayssa Dias Faleiro, Gabriela Pereira Salça de Almeida , Jessica Karoline de Oliveira Chaves, Diefrey Ribeiro Campos 2020-12-07 2020-12-07 42 1 e000820 e000820 10.29374/2527-2179.bjvm000820 Unusual pulmonary thromboembolism in a Dirofilaria immitis infected dog <p>&nbsp;The nematode <em>Dirofilaria immitis </em>and its endosymbiont <em>Wolbachia </em>are the agents of canine heartworm (HW) disease. The worm´s main habitats are the pulmonary arteries, which elicit inflammation and disorders of the coagulation process. Clinical signs vary according to multiple factors, including worm burden, individual reaction, and duration of infection. This report describes an unusual case of pulmonary thromboembolism secondary to HW infection. A 7-year-old female dachshund presented with a history of cough, dyspnea, and two syncope episodes. On physical examination, the oral mucosa was slightly discolored, the femoral pulse was weak, respiratory distress was present, and the abdomen distended. Echocardiography showed severe right atrioventricular and pulmonary artery enlargement and a large structure with high echogenicity in the artery. After the dog died naturally, the lungs and heart were examined. Only one worm was found in the right ventricle, and a large thrombus was found in the main pulmonary artery. Histologically, fibrin thrombi and fragments of parasites were present inside the pulmonary artery branches. These findings highlight the importance of a thorough clinical evaluation of HW patients and confirm that only a few worms can threaten an animal’s life. Therefore, veterinarians must enforce chemoprophylaxis once treatment is life threatening.</p> Bruno Alberigi Nathália Marques de Oliveira Lemos Bernardo Nogueira de Farias Elan Cardozo Paes de Almeida Flavya Mendes-de-Almeida Norma Labarthe Copyright (c) 2020 Bruno Alberigi, Nathália Marques de Oliveira Lemos, Bernardo Nogueira de Farias, Elan Cardozo Paes de Alemida, Flavya Mendes-de-Almeida, Norma Labarthe 2020-12-07 2020-12-07 42 1 e000320 e000320 10.29374/2527-2179.bjvm000320 Quadricuspid aortic valve and supracrystal interventricular communication in a Brazilian equestrian horse <p>A 13-year-old Brazilian warmblood gelding arrived at the Veterinary Hospital of the Federal University of Paraná presenting a murmur on cardiac auscultation. At physical evaluation, an IV/VI intensity holosystolic heart murmur was heard. Due to this clinical sign, an echocardiogram examination was performed, which showed supracrystal interventricular communication, quadricuspid aortic valve (QAV) and, in Doppler evaluation, aortic reflux was noted and turbulent flow. Similar cardiac anomalies have been described in humans, hamsters, dogs, cats and shrews. However, there is only one case report in a horse and in which the equine had QAV and ventricular septal defect (VSD). Despite the congenital heart defects, the animal had no obvious signs of heart failure.</p> Jéssica do Rocio Janiszewski Andressa Duarte Lorga Anny Raissa Carolini Gomes Lucimara Strugava Bruna Machado Amaral Rosa Cassiano Ricardo Rios Marlos Gonçalves Sousa Peterson Triches Dornbusch Copyright (c) 2020 Jéssica do Rocio Janiszewski, Andressa Duarte Lorga, Anny Raissa Carolini Gomes, Lucimara Strugava, Bruna Machado Amaral Rosa, Cassiano Ricardo Rios, Marlos Gonçalves Sousa, Peterson Triches Dornbusch 2020-12-01 2020-12-01 42 1 e112320 e112320 10.29374/2527-2179.bjvm112320 Reversible third-degree atrioventricular block in a Poodle-Toy with degenerative mitral valve disease and pyometra <p>Third degree atrioventricular block is a severe arrhythmia, being the most common&nbsp;&nbsp; indication for artificial cardiac pacing in dogs. Spontaneous reversion to normal sinus rhythm is very rare or exceptional. A 12-year-old Poodle-Toy was referred because of history of abdominal enlargement, anorexia, vomiting and weakness. Abdominal ultrasonography revealed large pyometra. Then, a six-lead electrocardiogram presented third-degree atrioventricular block, while transthoracic Doppler echocardiography showed myxomatous mitral valve disease, presence of mitral, tricuspid and aortic regurgitation, accompanied by mitral and tricuspid diastolic insufficiency. Two days after the ovariohysterectomy, a new electrocardiogram revealed spontaneous reversal to the sinus rhythm, while the diastolic reflux of mitral and tricuspid valves disappeared. The patient showed rapid clinical amelioration and eight months after surgery was still alive, on treatment for heart failure. As far as we know, this is the first canine case of third-degree heart block, secondary to endocardiosis and pyometra, which was spontaneously reverted to sinus rhythm after surgery.</p> Gustavo Luiz Gouvêa Almeida Marcelo Barbosa de Almeida Ana Carolina Mendes dos Santos Ângela Vargas Valéria Duarte Reis Copyright (c) 2020 Gustavo Luiz Gouvêa Almeida, Marcelo Barbosa de Almeida, Ana Carolina Mendes dos Santos , Ângela Vargas, Valéria Duarte Reis 2020-12-10 2020-12-10 42 1 e109920 e109920 10.29374/2527-2179.bjvm109920 Report of Candidatus Mycoplasma haematoparvum and Mycoplasma haemocanis canine infections in Massambaba restinga, Brazil <p>Tick-borne diseases are frequent in the southeastern section of Brazil. The most prevalent canine parasites diagnosed are <em>Ehrlichia canis, Babesia gibsoni, Babesia canis, </em>and <em>Anaplasma platys</em>, although <em>Mycoplasma haemocanis </em>and <em>Candidatus Mycoplasma haematoparvum </em>have also been detected in other regions of the country. Two clinically healthy dogs from a suburban area of the state of Rio de Janeiro had a history of being heavily infested with ticks and were examined at IDEXX Reference Laboratories, California for a tick panel check. One dog harbored DNA of <em>Candidatus Mycoplasma haematoparvum </em>and the other, the DNA of <em>Mycoplasma haemocanis. </em>These results reinforce the need for permanent monitoring for tick infestations and tick-borne parasites in southeastern Brazil, particularly considering the likely continuation of global climate changes that will contribute to the spread and increase of infections across the country.</p> Marcia Miranda Bruno Alberigi Flavya Mendes-de-Almeida Alexandre Bendas Nadia Almosny Jonimar Paiva Norma Labarthe Copyright (c) 2020 Marcia Miranda, Bruno Alberigi, Flavya Mendes-de-Almeida, Alexandre Bendas, Nadia Almosny, Jonimar Paiva, Norma Labarthe 2020-12-08 2020-12-08 42 1 e000420 e000420 10.29374/2527-2179.bjvm000420 Detomidine and xylazine, at different doses, in donkeys (Equus asinus) <p>The effects of detomidine and xylazine in northeasters donkeys were compared. Six animals participated, randomly, of all experimental groups: xylazine 10%, 0.8 mg/kg (xylazine group 0.8 - GX0.8), 1.0 mg/kg (GX1.0) and 1, 2 mg/kg (GX1.2); and detomidine 1%, 0.02 mg/kg (Detomidine group 0.02 - GD0.02), 0.04 mg/kg (GD0.04) and 0.06 mg/kg (GD0.06) intravenous. Heart rate (HR) and respiratory rate (RR), rectal temperature (RT), mean arterial pressure (MAP), blood glucose, sedation, analgesia, ataxia, arrhythmias and urination were monitored. Duration of sedation was greater in the groups in which detomidine was administered. The muzzle-soil distance significantly reduced in all groups five minutes after the sedative administration, remaining smaller for a longer time in the GD0.06. Ataxia in GX1.0 was greater than in GX0.08 and GX1,2, being equivalent to GD0,04 and GD0,06. Analgesia lasted 30 minutes in GD0.06 and 10 minutes in the others. There was reduction of HR in GD0.02 and GD0.04 and atrioventricular block. There was a reduction in TR in GX1,2, GD0.02, GD0,04 and GD0,06. RR decreased in all groups. MAP increased in GD0.02, GD0.04 and GD0.06 five, 20, 30 and 10 minutes after administration of detomidine, respectively. Hyperglycaemia occurred for 120 minutes in all animals receiving detomidine. The frequency of micturition was higher in GD0.06 (2.2 ± 0.8) than in GX0.8 (0.8 ± 0.4). In northern Brazil, detomidine and xylazine promote short duration analgesia and cardiorespiratory alterations, but detomidine causes a greater sedative, hypertensive and hypothermic effect.</p> Roberta Nunes Parentoni Fernanda Vieira Henrique Arthur Willian de Lima Brasil Rafaela Alves Dias Ana Lucélia de Araújo Araújo Rodrigo de Souza Mendes Emanuel Ferreira Martins Filho João Moreira da Costa Neto Almir Pereira de Souza Pedro Isidro da Nóbrega Neto Copyright (c) 2020 Fernanda Vieira Henrique, Roberta Nunes Parentoni, Arthur Willian de Lima Brasil, Rafaela Alves Dias, Ana Lucélia de Araújo Araújo, Rodrigo de Souza Mendes, Emanuel Ferreira Martins Filho, João Moreira da Costa Neto, Almir Pereira de Souza, Pedro Isidro da Nóbrega Neto 2020-01-23 2020-01-23 42 1 e100820 e100820 10.29374/2527-2179.bjvm100820 Intestinal and immunological histological parameters of broilers supplemented with commercial probiotic or fungi of the autochthonous microbiota <p>Objetivou-se avaliar a histomorfometria das porções intestinais de frangos de corte suplementados com probiótico, bem como parâmetros histológicos de órgãos vinculados ao sistema imune. O fungo avaliado <em>Rhizomucor</em> spp. é proveniente de amostras do conteúdo intestinal de frangos Cobb 500. Este foi selecionado <em>in vitro</em> quanto características probióticas e encaminhado para ensaio de desempenho. Foi utilizado um delineamento inteiramente ao acaso com 3 tratamentos e 7 repetições. Uma ave por unidade experimental aos 21 e 42 dias de idade foi sacrificada e, os órgãos foram amostrados, após fixação foram submetidos à desidratação e diafanização e, em seguida, analisados por microscopia óptica e submetidos à análise de histomorfometria e pesquisados pontos que indicavam lesão. Observou-se aos 21 dias que as aves pertencentes ao grupo probiótico formulado a base de <em>Rhizomucor </em>spp. apresentaram maior altura de vilo no duodeno. Também constatou-se um aumento significativo na altura de vilo do jejuno para o grupo controle e probiótico formulado a base de <em>Rhizomucor</em> spp.. Aos 42 dias observou-se, apenas diferença para profundidade de cripta com uso do probiótico comercial para duodeno. Referente as análises das alterações teciduais dos intestinos, baço e bursa aos 21 dias, cem por cento das aves recebendo o fungo <em>Rhizomucor</em> spp. não apresentaram alterações histológicas e aos 42 dias foram observadas alterações na bursa em dois animais. A inclusão do potencial probiótico fúngico <em>Rhizomucor</em> spp. na dieta de frangos de corte aos 21 dias de idade promove maior altura de vilo e aos 42 dias de idade não houve influência.</p> Flávia Oliveira Abrão Danne Kelle Siqueira Lima Moisés Sena Pessoa Copyright (c) 2020 Flávia Oliveira Abrão, Danne Kelle Siqueira Lima, Moisés Sena Pessoa 2020-01-23 2020-01-23 42 1 e101220 e101220 10.29374/2527-2179.bjvm101220 Ultrasonographic evaluation of the reproductive tract as predictor of pregnancy in Girolando heifers submitted to Timed Artificial Insemination <p>The objective of this study was to evaluate the influence of the degree of uterine and ovarian development on the pregnancy rate of Girolando heifers submitted to Timed Artificial Insemination (TAI). Fifty-six heifers, representing 100% of the category in the herd studied, were evaluated. The heifers were submitted to a gynecological evaluation, prior to hormonal treatment, with ultrasonography, in order to measure the diameter of the uterine horns and ovaries, as well as evaluate the presence of corpus luteum (CL) and antral follicles. According to the values found for the uterine and ovarian diameters, the animals were classified into scores from one to three. After the gynecological evaluation, all animals were submitted to TAI. There was no statistical difference (P&gt;0.05) in pregnancy rates between the different uterine and ovarian scores, as well as in relation to ovarian structures. However, a statistical difference (P&lt;0.05) was observed in the percentage of animals in anestrous within ovarian scores one, two and three (83.3%, 37.5% and 42.8%, respectively). The conclusion was that uterine and ovarian development did not interfere in the pregnancy rate of Girolando heifers in the herd studied.</p> Lara Nogueira Silenciato Joaquim Esquerdo Ferreira Samuel Rodrigues Bonamichi do Couto Otávia Reis e Silva Osvaldo Almeida Resende Andressa Ferreira da Silva Helcimar Barbosa Palhano Marco Roberto Bourg de Mello Copyright (c) 2020 Lara Nogueira Silenciato, Joaquim Esquerdo Ferreira, Samuel Rodrigues Bonamichi do Couto, Otávia Reis e Silva, Osvaldo Almeida Resende, Andressa Ferreira da Silva, Helcimar Barbosa Palhano, Marco Roberto Bourg de Mello 2020-01-23 2020-01-23 42 1 e104220 e104220 10.29374/2527-2179.bjvm104220 Study of the heart rate variability in dogs with brachycephalic syndrome submitted to rhinoplasty surgery <p>The aim of the present study was to evaluate the HRV in the brachycephalic races, before and after surgical correction of the stenosis of the nostrils, by means of prolonged electrocardiography, observing the excess of the parasympathetic stimulation regarding the occurrence of bradyarrhythmias and their clinical consequences, and possibility of improvement of the autonomic balancing after the treatment, increasing the quality and life expectancy of these patients. Sixteen dogs with nostril stenosis were included, where the analysis and progression of the HRC findings at different moments before the surgical procedure (day 0) and after the procedure were performed in two moments, 30 and 60 days. The technique of alavestibulplasty was performed and HRV analysis was recorded by means of prolonged electrocardiography. For this examination, the indexes were analyzed in the time domain. Regarding the clinical findings, there was an improvement in the evaluation of the heart and respiratory rate, as well as a reduction in parasympathetic activity, as shown in the bradyarrhythmias. Regarding the HRV indexes in the time domain, rMSSD and SDNN associated with mean heart rate findings, have brought strong indications that the reduction of parasympathetic stimulation is related to the reduction of HRV in these animals. The rMSSD is the HRV index in the domain of the time that early changed can be used as indicator of the increase of the parasympathetic activity caused by the Brachycephalic Syndrome</p> Mário dos Santos Filho Daniel Carvalho Hainfellner Nathália Marques de Oliveira Lemos Karen Denise da Silva Macambira Barbosa Macambira Juliana Silva Do Carmo Bruno Ricardo Soares Alberigi da Silva Alberigi Carolina do Valle Aben Athar Aguinaldo Francisco Mendes Junior Cristiano Chaves Pessoa da Veiga Ana Maria Barros Soares Paulo de Tarso Landgraf Botteon Julio Israel Fernandes Jonimar Pereira Paiva Copyright (c) 2019 Mário dos Santos Filho, Daniel Carvalho Hainfellner, Karen Denise da Silva Macambira Barbosa Macambira, Juliana Silva Do Carmo, Nathália Marques de Oliveira Lemos, Bruno Ricardo Soares Alberigi da Silva Alberigi, Cristiano Chaves Pessoa da Veiga, Ana Maria Barros Soares, Paulo de Tarso Landgraf Botteon, Jonimar Pereira Paiva, Julio Israel Fernandes 2020-01-23 2020-01-23 42 1 e104919 e104919 10.29374/10.29374/2527-2179.bjvm104920 Chemical immobilization of free-living capybaras (Hydrochoerus hydrochaeris) using ketamine-dexmedetomidine combination and a remote drug delivery system <p>Capturing wild capybaras for scientific projects, population control or medical interventions is a growing necessity. With this study, we intended to evaluate a Ketamine/Dexmedetomidine as a reversible chemical restraint in free-ranging synanthropic capybaras, seeking enhanced anesthetic and recovery characteristics while testing a specialized remote drug delivery system (RDDS). For this purpose, 18 adult capybaras (male n = 8; females n = 10) (67.3 ± 9.45 kg), prior to chemical restraint, were physically confined, subsequently darted intramuscularly with 9 mg kg<sup>-1</sup> ketamine and 0,005 mg kg<sup>-1</sup> dexmedetomidine. Post-intervention, 0,005 mg kg<sup>-1</sup> atipamezole, administered IM, was used as a reversal agent (RA), (n = 5). Anesthetic effects were recorded as latency period (LP I/first observed effects) (LP II /lateral recumbency plus time to handle the animal). Recovery time was divided into (R1/no RA, fully recovered/ready for release), (R2a/R2b, with RA, time to ambulant position plus time to full recovery/release, respectively). Vital signs were recorded at a 15-minute interval. GraphPad Prism 8.1.1 was used to perform unpaired t-test, with a p-value ˂ 0,05 considered significant. Results: Mean LP I: 3 ± 1 min.; LP II: 10 ± 2 min. Procedure duration: 49 ± 5 min. Recovery time without RA, (R1): 55 ± 15 min., compared to 18 min., with RA (R2a) to ambulant position (with severe discoordination), requiring additional time until full recovery (ready for release): ± 45 min (R2b). Total time, to release (R2a/b): mean ± SD = 67 ± 13.85 min. Concluding for clinical relevance that the association of Ketamine and Dexmedetomidine performed satisfactorily, providing effective sedation and analgesia, and relative short latency periods. Used RA did not shorten total recovery time significantly (P-value = 0,7328). Adverse effects such as the risk of acute cecal tympany, due to the lack of pre-anesthetic fasting, concurrent to collateral effects of injectable and volatile anesthetics on the motility of the digestive tract, and induced bradycardia/hyperthermia warrant extra caution. The employed RDDS provided reliable drug delivery under field conditions.</p> DEREK ROSENFIELD Mario Ferraro Claudia Igayara Silvia Renata Gaigo Cortopassi Cristiane Schilbach Pizzutto Copyright (c) 2020 DEREK ROSENFIELD, Mario Ferraro, Claudia Igayara, Silvia Renata Gaigo Cortopassi, Cristiane Schilbach Pizzutto 2020-04-17 2020-04-17 42 1 e107220 e107220 10.29374/2527-2179.bjvm107220 Methicillin-resistant Staphylococcus pseudintermedius: an underestimated risk at pet clinic <p>The prevalence of methicillin-resistant <em>Staphylococcus pseudintermedius</em> (MRSP) as a cause of infectious disease in companion animals remains unknown. The emergence of MRSP is a challenge in veterinary medicine as multidrug-resistant strains began to emerge, resulting in treatment failures. This study provides an overview of the characterization of <em>S. pseudintermedius</em> strains from clinical pet samples and the prevalence of MRSP strains. A total of 123 <em>S. pseudintermedius</em> strains were characterized by phenotypic testing and the MALDI-TOF technique and evaluated for susceptibility to methicillin and the presence of the <em>mec</em>A gene. Of these, 49 (39.8%) were identified as MRSP. The results confirm the importance of monitoring resistant pathogens and the need for further studies to determine the prevalence of MRSP in companion animals. The prevalence of methicillin-resistant <em>Staphylococcus pseudintermedius</em> (MRSP) as a cause of infectious disease in companion animals remains unknown. The emergence of MRSP is a challenge in veterinary medicine as multidrug-resistant strains began to emerge, resulting in treatment failures. This study provides an overview of the characterization of <em>S. pseudintermedius</em> strains from clinical pet samples and the prevalence of MRSP strains. A total of 123 <em>S. pseudintermedius</em> strains were characterized by phenotypic testing and the MALDI-TOF technique and evaluated for susceptibility to methicillin and the presence of the <em>mec</em>A gene. Of these, 49 (39.8%) were identified as MRSP. The results confirm the importance of monitoring resistant pathogens and the need for further studies to determine the prevalence of MRSP in companion animals.</p> Theresse Holmstrom Luria Adib David Cássia Couto da Motta Thomas Hebert dos Santos Irene da Silva Coelho Shana de Mattos de Oliveira Coelho Dayanne Araújo de Melo Miliane Moreira Soares de Souza Copyright (c) 2020 Theresse Holmstrom, Luria Adib David , Cássia Couto da Motta, Thomas Hebert dos Santos, Irene da Silva Coelho, Shana de Mattos de Oliveira Coelho, Dayanne Araújo de Melo, Miliane Moreira Soares de Souza 2020-07-01 2020-07-01 42 1 e107420 e107420 10.29374/2527-2179.bjvm107420 ANIMAL HEALTH SURVEILLANCE BY QUADRANT - A NEW "INTELLIGENT" AGRICULTURAL SURVEILLANCE SYSTEM <p>Brazil is the world's greatest beef exporter and Mato Grosso do Sul state is one of the most relevant states of Brazil with utmost importance for animal production. To ensure the health of the animal herd, the Livestock Defense services of all federative units perform securities activities, although not as effective as they should be, following the procedures of the Ministry of Agriculture, Livestock and Supply (MAPA). This study aims to present a brand new health surveillance system established in Mato Grosso do Sul state where each city was mapped in blocks and ranked by hazard levels. An activity planning is carried out in which all city blocks receive livestock defense surveillance. The actions are tracked and registered through GPS devices and the data are assessed by satellites images analyzer software. Therefore, the actions are auditable and the results monthly analyzed for future actions planning. As a result, the surveillance actions ceased to be performed on demand and started to cover not only the areas next to the local office but the entire city areas as well; the registration's and sanitary program's data were updated and activities had become clear and auditable, allowing all actions of the Health Defense services to be controlled and monitored. It is known that, once these actions are implemented in other federative units it may lead to a differential advantage to Brazilian Health Defense Services.</p> Francisco Bezerra de Carvalho Neto Franco Zanandreis Clayton Bernardinelli Gitti Copyright (c) 2020 Francisco Bezerra de Carvalho Neto, Franco Zanandreis, Clayton Bernardinelli Gitti 2020-05-19 2020-05-19 42 1 e108020 e108020 10.29374/2527-2179.bjvm108020 CORTICOSTEROIDS USE IN NEUROLOGY: HOW DOGS ARE REFERRAL TO A NEUROLOGY SERVICE? <p>Corticosteroids are drugs widely used in veterinary neurology due to their recognized analgesic and anti-inflammatory effects. However, their use in high doses and long terms may result in undesirable effects and disorders in other systems. This study describes the prevalence of dogs with neurological diseases referred to a veterinary neurology reference service with previous prescription of corticosteroids. For the retrospective study 284 medical records of dogs were evaluated in the period between August 2017 and April 2019. Of these, 194 (68%) patients already had medical care by another veterinary, and, 100 (52%) had previous prescription for the corticosteroid use. After the diagnosis, the dose was suspended in 28% of the cases, adjusted in 41% and maintained in 31%. Dogs with immunosuppressive doses prescription were the most affected, and the medication was adjusted or suspended in 71% and 21% of the cases, respectively. In conclusion, the therapy for neurological diseases is still strongly associated with the indiscriminate use of corticosteroids, without a dose correlation with the desired effect.</p> Gabriela Wacheleski Brock Maria Eduarda Lopes Fernandes Clarice Gonring Corrêa Anna Julia Rodrigues Peixoto Alex Gradowski Adeodato Daniel de Almeida Balthazar Marta Fernanda de Albuquerque da Silva Cássia Maria Molinaro Coelho Copyright (c) 2020 Gabriela Wacheleski Brock, Maria Eduarda Lopes Fernandes, Clarice Gonring Corrêa, Anna Julia Rodrigues Peixoto, Alex Gradowski Adeodato, Daniel de Almeida Balthazar, Marta Fernanda de Albuquerque da Silva, Cássia Maria Molinaro Coelho 2020-05-20 2020-05-20 42 1 e106520 e106520 10.29374/2527-2179.bjvm106520 Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro <p>The canine babesiosis has a worldwide distribution, its one of the most important tick-borne disease. It is endemic in Brazil, where the prevalence is increasing in several áreas. Typically, anemia and thrombocytopenia are found in dogs with babesiosis, such as hematological changes. The aim of this study was to evaluate the piroplasms prevalence and hematologic changes in dogs at Small Animals Veterinary Hospital of Universidade Federal Rural do Rio de Janeiro (UFRRJ), through the amplification of the 18S rRNA gene using Polymerase Chain Reaction (PCR) and genetic description using sequencing. Blood samples of 250 dogs were randomly selected. From all samples, nine (3.6%) were positive for <em>Babesia</em> spp. using conventional PCR. The sequencing was performed on products from PCR and the positives samples were identified as <em>Babesia vogeli.</em> The main hematological alterations were anemia (66.7%) and thrombocytopenia (89%). The piroplasm that most affect dogs in the region is <em>Babesia vogeli</em> and it can develop anemia and thrombocytopenia.</p> Renata Quintela Assad Eloy Seabra Junior Monique Moraes Lambert Carmen Beatriz Seti Correa Tatiana Didonet Lemos Helena Keiko Toma Fabio Barbour Scott Nadia Regina Pereira Almosny Copyright (c) 2020 Renata Quintela Assad, Eloy Seabra Junior, Monique Moraes Lambert, Carmen Beatriz Seti Correa, Tatiana Didonet Lemos, Helena Keiko Toma, Fabio Barbour Scott, Nadia Regina Pereira Almosny 2020-10-07 2020-10-07 42 1 e032120 e032120 10.29374/2527-2179.bjvm032120 Investigation of Chlamydia psittaci in pet birds of Uberlandia city, Minas Gerais, Brazil <p>Chlamydiosis is a public health important zoonosis, and its transmission to humans occurs mainly through contact with pet birds. These animals rank the second position in most popular pets in Brazil, where the occurrence of chlamydiosis is underestimated, although studies indicate that the disease is endemic in the country. Therefore, the present work aimed to investigate the occurrence of <em>Chlamydia psittaci</em> in 68 cockatiel chicks (<em>Nymphicus hollandicus</em>) of the city of Uberlandia, Minas Gerais, Brazil. Samples of choana, cloaca, and fresh fecal samples were collected for the detection of the bacteria by PCR test. The occurrence found was null. This work concluded that it is important to raise the awareness of owners and breeders about measures to prevent and control this disease, and that orienting them about the risks of overusing antibiotics is a public health issue.</p> Andressa Afonso Borges Daniel Amaral Gontijo Ana Carolina de Andrade Mello Cintra de Amorin Karinne Spirandelli Carvalho Naves Copyright (c) 2020 Andressa Afonso Borges, Daniel Amaral Gontijo, Ana Carolina de Andrade Mello Cintra de Amorin, Karinne Spirandelli Carvalho Naves 2020-10-15 2020-10-15 42 1 e107720 e107720 10.29374/2527-2179.bjvm107720 Influence of adult Ctenocephalides felis felis (SIPHONAPTERA, PULICIDAE) on the response to fipronil in vitro test <p><em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Ctenocephalides felis felis</span></span></em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;"> é um ectoparasita sifonáptero de grande importância na medicina veterinária e na saúde pública. </span><span style="vertical-align: inherit;">Este estudo teve como objetivo avaliar a influência de diferentes idades de pulgas adultas de C. felis felis na suscetibilidade ao fipronil quando submetidas a teste in vitro. </span><span style="vertical-align: inherit;">Pulgas adultas de </span></span><em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">C. felis felis</span></span></em><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">colônia de laboratório. </span><span style="vertical-align: inherit;">A metodologia utilizada foi a impregnação de papel de filtro com fipronil na concentração de 0,². </span><span style="vertical-align: inherit;">Oito grupos experimentais foram formados de acordo com a idade das pulgas (grupos 0, 7, 14 e 21 dias). </span><span style="vertical-align: inherit;">O critério de avaliação utilizado foi a motilidade, avaliada 24 e 48 horas após o desafio. </span><span style="vertical-align: inherit;">Após 24 horas, as pulgas com 21 dias de incubação tiveram uma taxa de mortalidade de 100%, que diminuiu com a idade. </span><span style="vertical-align: inherit;">As pulgas com 14 dias tiveram mortalidade de 98,3%, 7 dias 93,1% e 0 dias com 86,7%. </span><span style="vertical-align: inherit;">Dentro de 48 horas, o fipronil foi 100% eficaz em todas as idades. </span><span style="vertical-align: inherit;">Com base nos resultados, pode-se concluir que a idade das pulgas não influenciou a resposta do teste in vitro à suscetibilidade ao fipronil às 24 e 48 horas.</span></span></p> Renan Bernardes Tavares Gabriela Pereira Salça de Almeida Jessica Karoline Oliveira Chaves Diefrey Ribeiro Campos Thais Ribeiro Correia Azevedo Copyright (c) 2020 Renan Bernardes Tavares, Gabriela Pereira Salça de Almeida, Jessica Karoline Oliveira Chaves, Diefrey Ribeiro Campos, Thais Ribeiro Correia Azevedo 2020-10-22 2020-10-22 42 1 e107920 e107920 10.29374/2527-2179.bjvm107920 Responsible ownership for cats and dogs from different urban neighborhoods of the City of Umuarama, Paraná, Brazill <p>The article analyzed the responsible care and well-being of dogs and cats of different districts of the municipality of Umuarama, PR. We interviewed 53 tutors by means of a directed questionnaire, obtaining 106 animals evaluated indirectly. It was possible to determine several aspects related to the behavior of tutors and important data related to animal welfare, and the deficiency of reproductive control being an important factor verified in this study. It was concluded that the responsible care is not fully exercised by the population of Umuarama, making urgent the elaboration of public policies to improve this situation.</p> Thaís Camaso de Sá Jessé Lahos Borges Ana Maria Quessada Antônio Sérgio Ferraudo Eduardo Herrera Dias Marco Aurélio Cunha del Vechio Pollyana Linhares Sala Mayara da Silva Trentim Luciana Kazue Otutumi Copyright (c) 2020 Thaís Camaso de Sá, Jessé Lahos Borges, Ana Maria Quessada, Antônio Sérgio Ferraudo, Eduardo Herrera Dias, Marco Aurélio Cunha del Vechio, Pollyana Linhares Sala, Mayara da Silva Trentim, Luciana Kazue Otutumi 2020-11-18 2020-11-18 42 1 e105420 e105420 10.29374/2527-2179.bjvm105420 Reproductive efficiency and milk yield in Sindhi breed cattle <p>The aim of this study was to identify the average of productive and reproductive traits in Red Sindhi females and evaluate if there is an influence of the reproductive traits on milk yield in this breed. Data related to reproductive and productive traits of 638 Sindhi breed dairy cows from North, Northeast, Midwest and Southeast regions of Brazil were provided by ABCZ. Analysis of variance followed by analysis of correlation among reproductive traits and between these and milk yield traits were used. Averages for age at first calving (AFC), calving age (CA), calving interval (CI), reproductive efficiency (RE), dry period (DP), lactation period (LP),milk yield (MY), and total milk yield (TMY) were 549.83 ± 560.47 days; 1843.53 ± 739.99 days; 533.28 ± 206.66 days; 75.46 ± 19.95%; 248.28 ± 206.66 days; 263.22 ± 63.51 days; 7.08 ± 2.78 kg of milk. day-1 and 1875.28 ± 893.36 kg of milk.lactation-1, respectively. The traits AFC, calving order (CO), CA, CI, RE and LP were separated into categories and there was no effect on MY among different categories, while the CO showed positive correlation with LP. Therefore, it can be concluded that Red Sindhi breed dairy cows showed high averages for AFC and medium averages for MY, CI and RE.</p> Rafaella da Rocha Olivieri de Barros Raquel Rodrigues Costa Mello Sabrina Luzia Gregio de Sousa Kaliane Nascimento de Oliveira Jorge Carlos Dias de Sousa Elisa Cristina Modesto Marco Roberto Bourg de Mello Copyright (c) 2020 Raquel Rodrigues Costa Mello, Rafaella da Rocha Olivieri de Barros, Marco Roberto Bourg de Mello, Elisa Cristina Modesto, Sabrina Luzia Gregio de Sousa, Kaliane Nascimento de Oliveira 2020-11-16 2020-11-16 42 1 e105120 e105120 10.29374/DOI: 10.29374/2527-2179.bjvm105120 Determination of echocardiographic parameters in spider monkey (Ateles spp.) collectives in captivity sedated with ketamine and midazolam <p>Nonhuman primates have long been studied and managed in research centers, zoos, and farms, but there is still a lack of information regarding their illnesses. The present study aimed to establish the echocardiographic parameters of normality among primates of the genus Ateles (spider monkey) that are housed at the Zoo of the City of Rio de Janeiro – RIOZOO Foundation. Nine specimens of the same species were used, 5 females and 4 males, that were clinically healthy, young adults, with an approximate captive age of 2 to 3 years, and an average weight of 8.5 kg. The animals were physically restrained with a net and soon after, chemical containment was carried out by means of an association of ketamine hydrochloride at a dose of 8 mg per kg and midazolam at 0.5 mg per kg, intramuscularly. The animals were submitted to chemical restraint to establish the clinical evaluation, blood collection for complete blood count and biochemical measurements, urine collection for urinalysis, chest X-ray in the ventro-dorsal and latero-lateral positions, and electrocardiographic exams, with all the parameters within the normal range, in addition to echocardiographic examination, which became the means by which the study parameters were acquired. During this examination, the following values were evaluated: cardiac output, ejection fraction, final cardiac volume, heart rate, left atrial diameter, aorta diameter, ratio between the diameters of the left atrium and aorta artery, thickness of the interventricular septum in diastole, diameter of the left ventricle in diastole, thickness of the free wall of the left ventricle in diastole, thickness of the interventricular septum in systole, diameter of the left ventricle in systole, thickness of the free wall of the left ventricle in the systole, left ventricular end systolic volume, left ventricular end diastolic volume, septum bending fraction, fraction of curving of the posterior wall, septal separation, and shortening fraction. With the data obtained, tables were drawn with the echocardiographic values of the species studied under sedation.</p> Daniel De Almeida Balthazar Marcelo dos Santos Vasconcelos Bárbara Souza Neil Magalhães Andressa Kagohara Fernando Troccoli Alessandro Galhões Mário dos Santos Filho Jonimar Pereira Paiva Copyright (c) 2020 Daniel De Almeida Balthazar, Marcelo dos Santos Vasconcelos, Bárbara Souza Neil Magalhães, Andressa Kagohara, Fernando Troccoli, Alessandro Oliveira Galhões, Mario Santos Filho, Jonimar Pereira Paiva 2020-12-03 2020-12-03 42 1 e117120 e117120 10.29374/2527-2179.bjvm107120 Prevalence and risk factors of gastrointestinal parasites in sheep from Ubaté, Cundinamarca, Colombia <p>&nbsp;Sheep production is considered an important sector of meat supply for human consumption and contributes to development of the rural areas. Gastrointestinal infection in sheep include helminthic and coccidial infections and in order to establishing preventive measures against parasites in flocks, an understanding of its epidemiology is required. The aim of this study was to determine the prevalence of helminthic and coccidial infections in sheep from Ubaté - Colombia by Ritchie concentration method and to identify the risk factors (sex, feeding, age, physiological state and deworming condition) associated with infection. 400 fecal samples were collected using a convenience sampling method. Risk factors were analyzed by logistic regression analysis. Risk was expressed as an odds ratio (OR) with 95% confidence interval. Descriptive epidemiology and risk factors were performed using EpiInfo software. The overall prevalence was 78.1%. The different families observed were Dictyocaulidae (43.5%), Trichostrongylidae (32.7%), Ascaridae (2.8%), Trichuridae (3%), Chabertiidae (39.7%), Fasciolidae (0.4%), Eimeriidae (93.2%) and Taeniidae 0.2%. Silage feeding constitute a risk factor for the acquisition of coccidian (<em>p: </em>0.03). It is concluded that the prevalence of helminthic and coccidial infections in Ubaté municipality is high.</p> Adriana Maria Diaz Anaya Martin Orlando Pulido Medellin Ginette Isabel Chavarro Tulcan Copyright (c) 2020 Adriana Maria Diaz Anaya, Martin Orlando Pulido Medellin 2020-12-03 2020-12-03 42 1 e098819 e098819 10.29374/2527-2179.bjvm098819 Hematological and biochemical aspects of cats naturally infected with feline immunodeficiency virus and feline leukemia <p>Considering the importance and severity of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections, information on laboratory abnormalities can determine the prognosis of infected cats. This study aimed to determine the laboratory alterations of domiciled asymptomatics cats naturally infected by FIV and/or FeLV in Northeastern Brazil. Blood samples from 200 cats were evaluated by nested-PCR and commercial immunochromatographic test for diagnosis of these infections. Complete blood count (CBC) and serum biochemistry analyses were performed to evaluate laboratory abnormalities. CBC and biochemical values of cats tested positive for FIV and/or FeLV were tabulated for the presence or absence of changes and analyzed using the chi-square test with Yates correction or Fisher’s exact test for each variable, with a confidence interval of 95%. The total frequency was 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. The presence of hyperbilirubinemia (total, direct, and indirect) was the only change observed in cats positive for FIV compared to FIV-negative controls (p&lt;0.05). We believe that laboratory changes compatible with immunosuppressive conditions should be more frequent in FIV/FeLV positive cats that already present clinical signs of the disease.</p> Luciana Carvalho Lacerda Aísla Nascimento da Silva Rebeca Dálety Santos Cruz Jéssica de Souza Freitas Roueda Abou Said Alexandre Dias Munhoz Copyright (c) 2020 Luciana Carvalho Lacerda, Aísla Nascimento da Silva, Rebeca Dálety Santos Cruz, Jéssica de Souza Freitas, Alexandre Munhoz, Roueda Abou Said 2020-12-03 2020-12-03 42 1 e110020 e110020 10.29374/10.29374/2527-2179.bjvm110020 Performance and immune response of 1 to 21-day-old broilers fed diets supplemented with organic zinc <p>The objective of this study was to evaluate the effect of levels of zinc in organic form in diets for broilers in the period 1- 21 days on performance and immune response. Were underwent the experimental design completely randomized, with four treatments and five replicates. Treatments consisted of supplementing the diet with organic zinc at different levels (30, 60, and 90 mg/kg) plus an unsupplemented control treatment. The guaranteed levels of supplementary organic zinc (<em>carbon</em>-<em>amino</em>-<em>phospho</em>-<em>chelate</em>) were 100,000 mg/kg. In pre-starter phase, 1 a 7 days, the &nbsp;treatments had a quadratic effect on feed intake (P=0,02) and weight (P=0,06) according were respectively equations:&nbsp; y=-6E-06x<sup>2</sup>+0,0007x+0,1273 (R<sup>2</sup>=0,93) e y = -2E-06x<sup>2</sup> + 0,0002x+0,1085 (R²=0,76), where the highest values were respectively, in the additional levels of 58.33 and&nbsp; 50.0 mg of zinc/kg diet. The treatments affected of quadratic way the carcass yield and the relative weight of the pancreas, providing higher values in the levels of 47.33 and 75 mg / kg, 44 respectively. The relative heart weight was influenced positively linearly by treatments. It is concluded that supplementing the diet with organic zinc has effects on performance, influence carcass yield, circulatory system and endocrine system in broiler.</p> Sandra Regina Gomes Silva João Batista Lopes Daniel Medeiros Noronha Albuquerque Elvania Maria da Silva Costa Moreira Daniela Cristina Pereira Lima Mabell Nery Ribeiro Copyright (c) 2020 Sandra Regina Gomes Silva, João Batista Lopes, Daniel Medeiros Noronha Albuquerque, Elvania Maria da Silva Costa Moreira, Daniela Cristina Pereira Lima, Mabell Nery Ribeiro 2020-11-18 2020-11-18 42 1 e101120 e101120 10.29374/2527-2179.bjvm101120 Evaluation of transsurgical stress in bitches submitted to ovariosalpingohisterectomy under infusions of fentanyl, lidocaine, and ketamine, associated or not with dexmedetomidine <p>Ketamine and dexmedetomidine are analgesic drugs indicated to control surgical pain. This study analyzes the increase in cortisol and blood glucose levels in bitches undergoing continuous infusion of dexmedetomidine and ketamine in ovariosalpingohisterectomy. For that, we compared groups using these drugs with a group using FLK (fentanyl, lidocaine, and ketamine) and the control group (without transsurgical analgesia). The experiment included 40 young (3.8 ± 2.8 years) mixed-breed bitches with average body weight of 9.8 ± 2.4 kg. These animals were allocated into 5 groups according to the recommended anesthetic technique: GCO (control group for pain); GFLK (control group for analgesia), undergoing continuous infusion of fentanyl (50 μg/mL), lidocaine (150 mg), and ketamine (30 mg), or FLK diluted in 500 mL of 0.9% saline solution and infused, after fentanyl bolus (5 μg/kg), at a rate of 10 mg/kg/h, equivalent to: 0.03 μg/kg/min of fentanyl associated with 50 μg/kg/min of lidocaine and 10 μg/kg/min of ketamine, about 5 minutes before surgical incision; GCE, undergoing infusion of only ketamine at the time of surgery, at a rate of 10 μg/kg/min, after intravenous administration of this drug at 2 mg/kg; GDEX, undergoing infusion of dexmedetomidine (2 μg/kg diluted in 20 mL of 0.9% NaCl), starting with an infusion of 2 μg/kg for 5 minutes, followed by an infusion rate of 2 μg/kg/h; GDEXCE, which received dexmedetomidine bolus (2 μg/kg) plus ketamine (2 mg/kg diluted in 20 mL of 0.9% NaCl solution), intravenously, over a period of five minutes. Then, the alpha-2 agonist (diluted in 20 mL of 0.9% NaCl) was infused at a rate of 2 μg/kg/h, and ketamine was continuously infused at 10 μg/kg/min. The results showed a significant reduction of cortisol levels of animals in the GDEXCE group in comparison to the animals in the GCO group at moment 5 (M5). However, cortisol and blood glucose values after extubation were influenced by the action of drugs. Thus, cortisol and blood glucose are not considered effective parameters for assessing transsurgical stress in bitches submitted to ovariosalpingohisterectomy and anesthetized with dexmedetomidine plus ketamine. Considering hemodynamic and behavioral parameters, dexmedetomidine associated with ketamine reduced the surgical stress of animals in the GDEXCE group.</p> Kleyton Domingos de Melo Ana Paula Monteiro Tenório Eduardo Alberto Tudury Mauro de Araújo Penaforte Junior Sabrina Cândido Trajano Maria Sheila da Silva Ferreira Gabriela Ratis Galeas Karine Silva Camargo Copyright (c) 2020 Kleyton Domingos de Melo, Ana Paula Monteiro Tenório, Eduardo Alberto Tudury, Mauro de Araújo Penaforte Junior, Sabrina Cândido Trajano, Maria Sheila da Silva Ferreira, Gabriela Ratis Galeas, Karine Silva Camargo 2020-12-07 2020-12-07 42 1 e105320 e105320 10.29374/2527-2179.bjvm105320 Bacterial isolation and evaluation of antisepsis protocols of the operative field of bitches submitted to ovariohysterectomy <p><strong>&nbsp;</strong>This study evaluated the efficiency of five antisepsis protocols performed in the operative field of bitches due to the importance of the prevention of surgical infections in veterinary medicine. Thirty female bitches submitted to elective ovariohysterectomy (OVH) were used, separated into groups of eight animals. <br>In group I, 70% alcohol and 2% chlorhexidine gluconate (CHG) were administered in group II, alcoholic&nbsp; CHG 0.5%, in group III polyvinylpyrrolidone (PVPI) 10% alcohol, group IV alcohol 70% and alcoholic PVPI 10 %, and in group V, alcohol, PVPI 10% and CHG degermante 2%. Samples were collected with sterile swabs prior to antisepsis and three minutes after each product was used and seeded in specific medium for colony-forming unit (CFU) counts. The identification of the isolates was performed according to the morphological, dyeing and biochemical characteristics. Bacillus spp., Staphylococcus spp., Klebsiella pnemoniae, Klebsiella oxytoca, Shigella sonnei, Shigella spp., Enterobacter aerogenes, Enterobacter spp., Salmonella spp. and Escherichia coli. The results obtained allow us to conclude that the protocols which CHG 0.5% alcohol and CHG 2% degermante were the most efficient in antisepsis, reducing 100% of the CFU of the skin and presented better residual power until the end of the surgical procedure.</p> Sabrina Cândido Trajano Breno Bezerra Aragão Mauro de Araujo Penaforte Junior Kleyton Domingos de Melo Maria Sheila da Silva Ferreira Júnior Mário Baltazar de Oliveira Rinaldo Aparecido Mota Grazielle Anahy de Sousa Aleixo Copyright (c) 2020 Sabrina Cândido Trajano 2020-12-07 2020-12-07 42 1 e105920 e105920 10.29374/2527-2179.bjvm105920 Use of trigger tool to identify Adverse Drugs Events in dogs. <p>The highlight of the shortcomings in the regulation and surveillance of veterinary drugs in Brazil is the absence of a system to report adverse drug events (ADEs). The aim of this study was evaluate the use of triggers in dogs to detect ADEs by estimating the prevalence of ADE and identifying the possible triggers. We screened medical records for ADEs and degree of harm caused by an ADE, using known triggers, with the Global Trigger Tool: translated, adapted and validated for use in animal health. The triggers comprised two-fold increase in blood urea nitrogen or serum creatinine levels compared to baseline, administration of vitamin K, administration of diphenhydramine or promethazine, excessive sedation or hypotension, administration of an antiemetic, abrupt cessation of medication, or others. The association between the occurrence of ADEs and variables, such as age, race, duration of hospitalization, polypharmacy, and clinical outcome was evaluated. A total of 148 medical records were analyzed. The trigger tools identified the known triggers 109 times in 68 medical records. Additionally, 14 ADEs were identified; the prevalence of ADEs was 9.5%. Positive predictive values of the triggers were 12.8%, and “vitamin K administration” had the best performance. The duration of hospitalization (p-value = 0.030) and polypharmacy (p-value &lt; 0.001) were associated with the occurrence of ADEs. Approximately 46% of the hospitalized dogs presented with at least one trigger. One out of five hospitalized dogs suffered from temporary harm due to an ADE. The duration of hospitalization and polypharmacy were found to be risk factors for ADEs in dogs.</p> Beatriz Cristina de Oliveira Fonseca Marcela Forgerini Leila Crystina Dias Zorzin Marcelo Rodrigues Martins Patrícia de Carvalho Matroianni Angela Ferreira Lopes Paulo Henrique Jorge da Cunha Nathalie de Lourdes Souza Dewulf Copyright (c) 2020-12-08 2020-12-08 42 1 e109720 e109720 10.29374/2527-2179.bjvm109720 Carcass traits in broilers fed diets containing L-glutamine and organic zinc <p>The objective of this study was to evaluate the effect of L-glutamine and organic zinc in diets for broilers on the parameters of carcass and meat yields, chemical composition and nutrient deposition in poultry carcasses at 21 days of age. Ross chicks (n=630) were distributed in a 2 x 3 + 1 completely randomized design, using levels of 1 and 2% L-glutamine with 0, 90 and 120 mg organic zinc/kg feed and a control diet, totaling seven treatments and five repetitions, with 18 birds each. The combination of L-glutamine and organic zinc did not influence the parameters evaluated. However, zinc supplementation alone interfered in a linearly increasing way with breast yield, in a linearly decreasing way with abdominal fat, and protein content in the carcass. Still, independently, regarding the chemical composition of poultry carcass, the level of 2% L-glutamine provided a higher concentration of dry matter and ash. Likewise, it showed the highest ash deposition. Supplementation of 2% L-glutamine in broiler diets improves the dry matter and the ash content in poultry carcasses and the inclusion of organic zinc positively interferes with the yield of breast, abdominal fat and crude protein</p> Daniela Cristina Pereira Lima Elvania Maria da Silva Costa Moreira Sandra Regina Gomes da Silva Mirian Lima Fernandes Mabell Nery Ribeiro Tatiele Pereira Araújo Jackelline Cristina Ost Lopes Jefferson Douglas Martins Ferreira João Batista Lopes Agustinho Valente de Figueiredo Copyright (c) 2020 Daniela Cristina Pereira Lima, Elvania Maria da Silva Costa Moreira, Sandra Regina Gomes da Silva, Mirian Lima Fernandes, Mabell Nery Ribeiro, Tatiele Pereira Araújo , Jackelline Cristina Ost Lopes, Jefferson Douglas Martins Ferreira , João Batista Lopes , Agustinho Valente de Figueiredo 2020-12-08 2020-12-08 42 1 e113320 e113320 10.29374/2527-2179.bjvm113320 Effects of pure and ozonated sunflower seed oil (Helianthus annuus) on hypergranulation tissue formation, infection and healing of equine lower limb wounds <p>This study was undertaken to evaluate the therapeutic effects of topical application of pure sunflower seed oil (oil group) and ozonated sunflower seed oil (ozone group) on acute cutaneous wound healing in eight healthy horses. The control group was treated with 0.9% sodium chloride. Two wounds were surgically produced on each horse on the proximal epiphysis of the metacarpus on the dorsal aspect of each forelimb. Wound area and contraction were measured on days 0, 3, 7, 14 and 21. Biopsy samples were taken to evaluate epithelial hyperplasia, inflammation, exuberant granulation tissue, exudate type, angiogenesis, and fibroplasia. Culture swabs were also collected. Wound contraction at 21 days for the oil group was 52.68%, for the ozone group was 72.16%, and for the control group (0.9% sodium chloride) was 34.80%. The ozone group had a significantly smaller wound size and a residual wound area than the control and the oil groups, on days 14 and 21. The control-wounds and oil-wounds had suppurative exudate and the presence of Streptococcus zooepidemicus. Neutrophilic inflammatory infiltrate was observed in all groups. Granulation tissue was observed on day 7 in all groups, but exuberant granulation tissue was observed only in the control group on days 14 and 21. Reepithelialization was observed on day 14 in the ozone group. In conclusion, these results demonstrate that topical application of ozonated sunflower seed oil accelerates acute cutaneous wound repair in horses, preventing hypergranulation tissue and infection, and that it is superior to treatment with pure sunflower seed oil and 0.9% sodium chloride.</p> Paula Alessandra Di Filippo Luiza Maria Feitosa Ribeiro Francielli Pereira Gobbi Gabriela Bravim Lemos Rachel Bittencourt Ribeiro Hassan Jerdy Larissa Carvalho da Silva Inácio Silva Viana Célia Raquel Quirino Copyright (c) 2020 Paula Alessandra Di Filippo, Luiza M.F. Ribeiro, Francielli P. Gobbi, Gabriela B. Lemos, Rachel Bittencourt, Hassan Jerdy, Larissa C. Silva, Inácio S. Viana, Célia R. Quirino 2020-12-08 2020-12-08 42 1 e113520 e113520 10.29374/2527-2179.bjvm1115321 Antimicrobial Potential of anaconda body fat oil (Eunectes murinus), in Santarém city, Pará state, Brazil <p>The use of natural substances from animals and plants has always been the subject of research, because it is made in a rustic way and through popular knowledge, built by everyday experiences and without scientific approval. The oil from the body fat of the snake - or better known as lard of anaconda, extracted from the anaconda snake (<em>Eunectes murinus</em>) is a product widely used in traditional communities and is marketed in markets for therapeutic purposes in several diseases. Because of the low cost and popular beliefs, many people use zootherapeutic means to treat themselves. Hence the importance of therapeutic proof of these products. Thus, the aim of this study was to verify whether the lard of anaconda made in the traditional way has antimicrobial potential in certain strains of pathogenic bacteria and fungi. For that, the evaluation of the lard’s antimicrobial potential was carried out using the disk diffusion method, adapted from the manual M7-A6 standardized by the <em>Clinical and Laboratory Standards Institute</em>, against the strains of <em>S. aureus, E. coli, Ç. albicans </em>and <em>C. krusei</em>. The observed results showed that there was no presence of bacterial or yeast growth inhibition halos, indicating that the anaconda lard oil did not inhibit the growth of fungi and bacteria in any of the strains used. Therefore, the present study allows us to conclude that the anaconda lard oil (<em>Eunectes murinus</em>), did not present any inhibitory antimicrobial potential, against the tested bacterial and fungal strains.</p> Lidiane Nascimento Araújo Christian Hermes Ribeiro Batista Osvaldo Gato Nunes Neto Hipócrates de Menezes Chalkidis Welligton Conceição da Silva Copyright (c) 2020 Lidiane Nascimento Araújo, Christian Hermes Ribeiro Batista, Osvaldo Gato Nunes Neto, Hipócrates de Menezes Chalkidis, Welligton Conceição da Silva 2020-12-01 2020-12-01 42 1 e112920 e112920 10.29374/2527-2179.bjvm112920 Mesenchymal Stromal/Stem cells: Historical perspective and ongoing challenges <p>The potential of stem cell-based therapy was accompanied by high expectations and led, consequently, to an increase in clinical translational investments. Mesenchymal stem/stromal cells (MSCs) became the cell-based product most experimentally studied worldwide. However, uncertainties about the mechanisms and the in vivo identity of MSCs impose concerns about their large-scale use in regenerative medicine. This review comprises a historical summary on MSC, a critical discussion about their identity and function, and the ongoing challenges for their use in therapy and tissue bioengineering.</p> Maria Isabel Doria Rossi Danielle Cabral Bonfim Copyright (c) 2020 Maria Isabel Doria Rossi, Danielle Cabral Bonfim 2020-06-11 2020-06-11 42 1 e112020 e112020 10.29374/2527-2179.bjvm112020