Brazilian Journal of Veterinary Medicine http://rbmv.org/index.php/BJVM <pre id="tw-target-text" class="tw-data-text tw-ta tw-text-small" dir="ltr" data-placeholder="Tradução" data-fulltext=""><span lang="en"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">O Jornal Brasileiro de Medicina Veterinária (BJVM) é o jornal oficial da Sociedade de Medicina Veterinária do Rio de Janeiro (Somverj). <br></span><span style="vertical-align: inherit;">Como uma política editorial, publica artigos científicos originais, revisões (no convite do Editor) e relatos de casos. <br></span><span style="vertical-align: inherit;">Em todos os campos da Medicina Veterinária. </span></span><br></span></pre> Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. en-US Brazilian Journal of Veterinary Medicine 0100-2430 Campylobacter spp. isolates of swine feces submitted to transport stress: species and antimicrobial resistance http://rbmv.org/index.php/BJVM/article/view/865 <p>The influence of transport stress in the presence of Campylobacter spp. and the antimicrobial resistance&nbsp;profile were evaluated in feces of 60 pigs. The samples were collected at the finishing farm and after transport&nbsp;to the slaughterhouse, totaling 120 samples. Isolation was performed by plaque culture and identification&nbsp;of the species was obtained by biochemical tests confirmed with the PCR technique. Campylobacter spp.&nbsp;was isolated in 63.3% of the collected samples at the farm and 91.6% at the slaughterhouse, evidencing the&nbsp;influence of transport stress on the increase of the isolates (P&lt;0.05). The species C. coli biotype I, C. jejuni&nbsp;biotype I and C. jejuni subsp. doylei were identified, with C. coli being more prevalent on the farm and&nbsp;C. jejuni in the slaughterhouse. Bacterial resistance was observed for all six classes of antibiotics tested.&nbsp;&nbsp;Among them, the isolates presented greater resistance to lincomycin, tetracycline and nalidixic acid&nbsp;(98.9%), and greater sensitivity to amoxicillin (22.5%). The strains of C. coli showed higher antimicrobial&nbsp;resistance than those of C. jejuni (P&lt;0.05). The results of this study point to the high isolation rates of&nbsp;C. coli and C. jejuni in pig feces destinated for slaughter and possible risks related to meat&nbsp; consumption.&nbsp;&nbsp;The high standards of resistance address the risk to public health.&nbsp;&nbsp;</p> Roberta Torres Melo Mônica Ribeiro Gabriel Eliane Pereira Mendonça Jéssica Laura Miranda Peixoto Guilherme Paz Monteiro Phelipe Augusto Borba Martins Peres Edson Campos Valadares Junior Denise Aparecida Rossi Copyright (c) 2019 Roberta Torres Melo https://creativecommons.org/licenses/by/4.0 2019-04-09 2019-04-09 41 e086519 e086519 10.29374/2527-2179.bjvm086519 Estudo da variabilidade da frequência cardíaca, por meio de eletrocardiografia contínua, em cães obesos, antes e após dieta terapêutica para emagrecimento. http://rbmv.org/index.php/BJVM/article/view/981 <p>O sistema nervoso autônomo (SNA) exerce papel de regulação do sistema cardiovascular por meio da modulação simpática e parassimpática, que atuam de maneira associadas na regulação da homeostase (Pumprla et al. 2002). Este balanço exercido pelo SNA é determinado por inúmeros fatores incluindo a respiração, estresse, temperatura, pressão sanguínea e atividade física (Vanderlei et al. 2009). A regulação nervosa cardíaca sofre influência da obesidade, mas ainda não foi esclarecida qual a resposta autonômica presente neste distúrbio (Pascon 2009). Sabe-se que o aumento de 10% da gordura corpórea é capaz de levar à diminuição da ação parassimpática e aumento da frequência cardíaca (Van Vliet et al. 1995). A Variabilidade da Frequência Cardíaca (VFC) permite avaliar a modulação autonômica sobre o coração, por meio dos intervalos de despolarizações ventriculares, ao eletrocardiograma (ECG), e fornece informações sobre a relação e contribuição do SNA na influência neural do controle da frequência cardíaca (Kleiger et al. 2005). O presente estudo tem por objetivo avaliar a VFC, por meio de exame eletrocardiográfico, em cães obesos, com Escore de Condição Corporal (ECC) 8/9 e 9/9, comparando com a VFC dos mesmos animais, após terapia com ração terapêutica no período de 6 meses.</p> Mário dos Santos Filho Daniel Carvalho Hainfellner Nathália Marques de Oliveira Lemos Camila de Oliveira Ferreira Mendes Patrícia Malandrim Jenifer Marques Campos Sophie Ballot Priscila Cardim de Oliveira Jonimar Pereira Paiva Copyright (c) 2019 Mário dos Santos Filho, Daniel Carvalho Hainfellner, Nathália Marques de Oliveira Lemos, Camila de Oliveira Ferreira Mendes, Patrícia Malandrim, Jenifer Marques Campos, Sophie Ballot, Priscila Cardim de Oliveira, Jonimar Pereira Paiva https://creativecommons.org/licenses/by/4.0 2019-04-10 2019-04-10 41 e98119 e98119 10.29374/2527-2179.bjvm98119 Diagnóstico, aspectos clínicos e epidemiológicos de bovinos leiteiros naturalmente infectados por Tripanosoma vivax nos estados de Pernambuco e Alagoas, Brasil. http://rbmv.org/index.php/BJVM/article/view/943 <p><em>Trypanosoma vivax</em> is the most pathogenic ruminant’s hemoparasite, causing huge economic losses to the producer when prevalent in the herd. This study aims to characterize the trypanosomiasis in naturally infected cattle in order to assess the clinical findings, epidemiological risk factors and diagnosis of this disease in outbreaks occurred in the states of Pernambuco and Alagoas, northeastern Brazil.&nbsp; For this purpose, historical and clinical examinations, blood collection and clinical monitoring were performed in 109 animals presenting illness for a period of one year. The main clinical findings were fever, apathy, anorexia, diarrhea, progressive weight loss, lymphadenopathy, pale mucous, incoordination, aggressiveness, abortion, decrease in milk production and high mortality. Out of 109 animals, 94% (103/109) were seropositive to <em>Trypanosoma vivax</em> by ELISA, 92% (100/109) were seropositive for IFAT, 15% (17/109) were positive in conventional PCR assay based on cathepsine L gene, and 9% (10/109) were showed the presence of <em>Trypanosoma</em> <em>vivax</em> trypomastigotes in stained-blood smears. In 13 samples, blood counts revealed that 69.23% (9/13) showed normocytic normochromic anemia and 53.84% (7/13) had leukocytosis, in 46.15% (6/13) neutrophilia and shift left regenerative. In 72 samples also examined whether it hematocrit, plasma protein and fibrinogen. Hematocrit presented average 22% (10% to 37%) to plasma protein had a mean value of 7.55 g/dl (5.4 g/dl to 10.0 g/dl) and plasma fibrinogen had a mean value 700mg/dl (200mg/dl to 1600mg/dl). The sequencing of DNA samples revealed 100% identicalness to <em>T. vivax </em>by BLAST analysis<em>.</em> The main factors involved in the spread of the disease were: the ingress of animals without prior tests and quarantine in the properties, application of oxytocin with sharing needles contaminated by blood at the time of lactation and the restriction of the disease in the lactation herd. The transmission of <em>T. vivax</em> occurred with the sharing of needles among animal’s lactation during application of oxytocin after the entry of carrier animals in the herd. Serological tests demonstrated a high rate of seropositive animals, however, conventional PCR and blood smear revealed a low rate positive animal’s because the animals were already being treated with trypanocide drugs. It is concluded that trypanosomiasis is a disease to be considered in the cattle regions studied as the cause of outbreaks. Hygienic and security actions must be taken during the administration of&nbsp;oxytocin in lactating cows. In fact, this practice, when carried incorrectly and without hygienic criterions and sharing needles, may play a role as the main risk factor in the transmission of trypanosomiasis in dairy herds among regions where the disease is prevalent, increasing the challenge of reinfection of animals.</p> Adony Querubino de Andrade Neto Carla Lopes de Mendonça Rodolfo José Cavalcanti Souto Paulo Henrique Sampaio Otávio Luiz Fidelis Junior Marcos Rogério André Rosangela Zacarias Machado Jose Augusto Bastos Afonso Copyright (c) 2019 Adony Querubino de Andrade Neto, Carla Lopes de Mendonça, Rodolfo José Cavalcanti Souto, Paulo Henrique Sampaio, Otávio Luiz Fidelis Junior, Marcos Rogério André, Rosangela Zacarias Machado, Jose Augusto Bastos Afonso https://creativecommons.org/licenses/by/4.0 2019-04-13 2019-04-13 41 e094319 e094319 10.29374/2527-2179.bjvm094319 Prevalence of gastrointestinal parasites in pet dogs and factors determining it, in urban area http://rbmv.org/index.php/BJVM/article/view/1001 <p>&nbsp;The aim of present study was to evaluate the prevalence of gastrointestinal parasite infection and related factors that govern prevalence in pet dogs. Of 200 fecal samples screened, a prevalence of 39% was observed, in which <em>Toxocara canis </em>(37%) and coccidian (29%) were predominant. Factors that could be associated with an increase in the probability of parasite detection in fecal samples included time since the last deworming, sex, and breed. Pet dogs with &gt; 6 months since the last deworming had a 1.5 times higher probability (P&lt;0.05) of gastrointestinal parasite infection, whereas purebred and males tended to have (P&lt;0.10) 0.9 and 0.8 times higher probability of gastrointestinal parasite infection than crossbreed and female dogs, respectively. In conclusion, results suggested that animals with &gt; 6 months since last deworming, purebred, and male dogs exhibited a comparatively higher prevalence and probability of gastrointestinal parasite infection.</p> LISSET LOYOLA-SUAREZ ADRIAN GUZMAN-SANCHEZ NORMA ANGELICA SERRANO-AGUILAR SILVIA GUADALUPE ESTRADA-BARRON ANA MARIA ROSALES-TORRES JOSE ANTONIO HERRERA BARRAGAN Copyright (c) 2019 LISSET LOYOLA-SUAREZ, ADRIAN GUZMAN-SANCHEZ, NORMA ANGELICA SERRANO-AGUILAR, SILVIA GUADALUPE ESTRADA-BARRON, ANA MARIA ROSALES-TORRES, JOSE ANTONIO HERRERA BARRAGAN https://creativecommons.org/licenses/by/4.0 2019-04-17 2019-04-17 41 e100119 e100119 10.29374/2527-2179.bjvm100119 The Epidemiological, clinicopathological, and economic aspects of an outbreak of botulism in beef cattle in the State of Tocantins, Brazil http://rbmv.org/index.php/BJVM/article/view/1019 <div>The objective of this work was to quantify the economic losses caused by a botulism outbreak among</div> <div>beef cattle in a semi-containment regime. The animals were fed corn silage and commercial feed and had</div> <div>access to a pasture of Guinea grass (</div> <div>Panicum maximum</div> <div>). Of the 150 steers in the herd, 22 (14.6% morbidity</div> <div>rate) presented clinical signs compatible with botulism and died (100% lethality rate). The 22 steers</div> <div>were clinically evaluated and eight were necropsied. The carcasses were evaluated macroscopically, and</div> <div>samples of the main organs were collected for laboratory testing. Ruminal and intestinal contents, and</div> <div>samples of the silage and animal feed provided were also collected. Botulinum toxin type C was identified</div> <div>in the ruminal contents of one steer and in the corn silage. Herein, the epidemiology, clinical signs, and</div> <div>laboratory findings regarding botulinum intoxication are emphasized for this outbreak. Our findings show</div> <div>the importance of keeping the silage well preserved because this represented a means of neurotoxin</div> <div>transmission that generated economic damage to the producer.</div> Samara de Paula Lopes Michel Abdalla Helayel Adriano Tony Ramos Isabelle Magalhães da Cunha Luiz Filipe Cabral de Souza Ramos Iveraldo dos Santos Dutra Vivian de Assunção N. Carvalho Saulo Andrade Caldas Copyright (c) 2019 Samara de Paula Lopes, Michel Abdalla Helayel, Adriano Tony Ramos, Isabelle Magalhães da Cunha, Luiz Filipe Cabral de Souza Ramos, Iveraldo dos Santos Dutra, Vivian de Assunção N. Carvalho, Saulo Andrade Caldas https://creativecommons.org/licenses/by/4.0 2019-04-23 2019-04-23 41 e101919 e101919 10.29374/2527-2179.bjvm101919 Factors associated with the seroprevalence of Neospora caninum (Apicomplexa: Toxoplasmatinae) in sheep from the State of Sergipe, Brazil http://rbmv.org/index.php/BJVM/article/view/28 <p>The aim of this study was to determine the prevalence and factors associated with the occurrence of antibodies against Neospora caninum in sheep from Sergipe, northeastern Brazil. A total of 932 sheep serum samples from 54 properties in 19 municipalities from the State of Sergipe, Brazil were collected and assayed using an indirect fluorescent antibody test (IFAT) to assess antibodies against N. caninum. A cut-off point of 1:50 was adopted and results showed that 12.45% (116/932) of sheep were serum-reactive. Based on an unconditional logistic regression, the presence of dogs on the property was associated with protection (OR= 0.323), whereas the use of exchanged or borrowed breeding males was associated with infection (OR= 22.287). These results indicate that the occurrence of antibodies against N. caninum is endemic in the State municipalities</p> Carlos Eduardo Dalencar Mendonça Alexandre Dias Munhoz Danielle de Santana Rocha Luciana Afonso Guimarães Rodrigo Alves Bezerra George Rego Albuquerque Cristiano Barros de Melo Copyright (c) 2019 Alexandre Dias Munhoz, George Rego Albuquerque, Cristiano Barros de Melo https://creativecommons.org/licenses/by/4.0 2019-05-22 2019-05-22 41 e002819 e002819 10.29374/2527-2179.bjvm002819 Orthopedic injury in 156 equids in Federal District, Midwestern Brazil http://rbmv.org/index.php/BJVM/article/view/956 <p>The present study aimed to establish the prevalence of orthopedic injuries and main clinical-epidemiological findings in equids referred to the Large Animal Veterinary Teaching Hospital of the Universidade de Brasília (HVET-UNB), during a 2-year period (March 2016 to February 2018). All equids records during the 2-year period were reviewed to select the orthopedic injury cases. Animal records were divided into two groups: traction animals and those participating in other equestrian activities. Definitive diagnosis, reached by means of physical evaluation and ancillary diagnostic (radiography and ultrasonography) methods, showed that 34% (156 cases) of the referred 438 equids, presented some sort of orthopedic disorders in the two-year period. Of these cases, 151 (96.8%) were horses, four (2.6%) mules, and one (0.6%) donkey. Ninety (58%) were traction horses and 66 (42%) participated in other equestrian activities, while 45.5% (71/156) were females and 54.5% (85/156), males. Forelimbs were the most affected (51.9% - 81/156), followed by hind limbs (41.1% - 64/156) and vertebral column disorders (7% - 11/156). Furthermore, 75% (117/156) presented some degree of lameness while 25% (39/156) had no pain or gait alterations. The three major orthopedic injuries were bone disorders (40.4% - 63/156), tendinopathies (25.6% - 40/156) and arthropathies (13.5% - 21/156) while the digital (9% - 14/156), muscle (6.4% - 10/156) and ligament (5.1% - 8/156) injuries were observed less frequently. Total mortality rate reached 32.7% (51/156), and fractures represented the major orthopedic disease leading to euthanasia (80.4% - 41/51). The high number of traction equids attended (90 animals - 58%) indicates the still frequent use of these animals in large urban centers. The high mortality rate in this category (50% - 45/90), representing 90.1% (45/51) of all deaths, reveals the need for developing public policies prohibiting horses from circulating in urban areas while also improving their welfare in the rural area. We reiterate the importance of retrospective studies for identifying risk factors, proposing management changes and creating policies to avoid animal suffering and financial losses.</p> Igor Louzada Moreira Rita Cássia Campebell Antonio Raphael Teixeira-Neto Antonio Carlos Lopes Câmara Copyright (c) 2019 Igor Louzada Moreira, Rita Cássia Campebell, Antonio Raphael Teixeira-Neto, Antonio Carlos Lopes Câmara https://creativecommons.org/licenses/by/4.0 2019-06-25 2019-06-25 41 e095619 e095619 10.29374/2527-2179.bjvm095619 Evaluation of canine leishmaniasis and concomitant seropositivity for Babesia canis and rickettsia in a nonendemic area in the central west region of Minas Gerais http://rbmv.org/index.php/BJVM/article/view/1018 <p>Among the infections caused by arthropods in dogs are <em>Leishmania infantum</em>, <em>Babesia canis</em> and rickettsias<em> Ehrlichia canis</em> and <em>Anaplasma phagocytophilum</em>. Studies show, in Brazil, high rates of co-infection in dogs by these agents. In Carmo da Mata, Central West of Minas Gerais there are no reports of cases of human or canine visceral leishmaniasis (CVL). In this municipality, the existence and prevalence of LVC and concomitant seropositivity with <em>B. canis</em> and rickettsias were evaluated. In the urban area, 433 domiciled dogs were tested for <em>Leishmania</em> infection, with three immunological tests (rapid immunochromatography and ELISA), with a prevalence of 0.7% (95% CI: 0.2-1.9). To evaluate the concomitant seropositivity, 433 dogs were evaluated by indirect immunofluorescence reaction, with 166 (38.3%) reagents for <em>A. phagocytophilum</em>, 171 (39.5%) for <em>B. canis</em> and 231 (53.3%) for <em>E. canis</em>. Of the total, 192 (44.2%) presented concomitant seropositivity with the three agents. Of the three dogs with CVL, one had only CVL and two had concomitant seropositivity, one with <em>B. canis</em> and the third with <em>A. phagocytophilum</em> and <em>E. canis</em>. Immunosuppression caused to dogs by ehrlichiosis can lead to an increase in cases of LVC and thus, it is necessary preventive measures to avoid the expansion of LVC in the municipality.</p> Marcella Oliveira Gama-Melo Bruna Torres Silvestre Julia Angélica Gonçalves Silveira Talita Pereira Vaz José Ronaldo Barbosa Múcio Flávio Barbosa Ribeiro Gilberto Fontes Copyright (c) 2019 Gilberto Fontes https://creativecommons.org/licenses/by/4.0 2019-05-10 2019-05-10 41 e101819 e101819 10.29374/2527-2179.bjvm101819 M-mode echocardiography measurements of healthy Brazilian random bred cats http://rbmv.org/index.php/BJVM/article/view/1029 <p>To determine the M-mode echocardiography reference measurements for Brazilian random bred cats in Rio de Janeiro, Brazil, Cats presenting for routine care were examined by M-mode echocardiography. Animals with free wall values ​​of the left ventricle and/or interventricular septum above 5 mm were not included. A total of 125 cats were included in the study. The correlation of the body score condition (BSC) with the echocardiography measurements was positive for left ventricle wall in systole (LVWs)&nbsp; and left ventricular wall in diastole (LVWd) . The LVWd values of BSCs 3 and 4 were different , and those of BSCs 3 and 5 were also different. The LVWs values of BSCs 3 and 5 were different . The correlated effect between BSC and the echocardiography measurements was true for the left atrium , left atrium/aorta ratio, ventricular septum in diastole, ventricular septum in systole, LVWs, LVWd, left ventricle in diastole, left ventricle in systole, ejection fraction and shortening fraction. The M-mode echocardiography evaluation of cats is impacted by the animal’s body mass; therefore, the BSC must be taken into consideration when interpreting echocardiography measurements.&nbsp;</p> Bruno Alberigi Alexandre José Rodrigues Bendas Denise do Vale Soares Carolina Aben Athar Fabiana Knacfuss Batalha Renan Salhab Demo Jonimar da Silva Paiva Copyright (c) 2019 Bruno Alberigi, Alexandre José Rodrigues Bendas, Denise do Vale Soares, Carolina Aben Athar, Fabiana Knacfuss Batalha, Renan Salhab Demo, Jonimar da Silva Paiva, Deceased https://creativecommons.org/licenses/by/4.0 2019-06-26 2019-06-26 41 e102919 e102919 10.29374/2527-2179.bjvm102919 Comparative study of the clinical and echodopplercardiographic aspects of left ventricular hypertrophy and hypertrophic cardiomyopathy in cats (Felis catus). http://rbmv.org/index.php/BJVM/article/view/1034 <p><strong>&nbsp;</strong>The aim of the present study was to differentiate hypertrophic cardiomyopathy (HCM) from concentric left ventricular hypertrophy (CLVH) and to compare their echodopplercardiography measurements in random bred domestic cats. After owners consent cats of any sex or age with no history of heart disease were randomly submitted to physical examination and echocardiogram. When left ventricular hypertrophy was present on the echocardiogram, cats were further examined by chest X-rays, ultrasonography and laboratory work. Those presenting cardiac hypertrophy with the diagnosis of any disease that could cause left ventricular hypertrophy were allocated into one group (CLVH) and those presenting hypertrophy without any concomitant detectable disease were allocated to another group (HCM). Cats with ventricular hypertrophy cats were included (n=10), among which five were classified as secondary CLVH, with hyperthyroidism being the main cause and five characterized as HCM. Considering the diagnosis of concentric ventricular hypertrophy, other diseases should be investigated and ruled out, such as hyperthyroidism. It is also necessary to consider and monitor cardiac changes more closely, since their phenotypic manifestation was severer than those observed in the animals with HCM. However, to determine whether disease progression in these animals is faster severer than in others, further epidemiological studies are necessary.</p> Bruno Alberigi Alexandre Bendas Denisa Soares Renan Demo Mateus Almeida Jonimar Paiva Copyright (c) 2019 Bruno Alberigi, Alexandre Bendas, Denisa Soares, Renan Demo, Mateus Almeida, Jonimar Paiva http://creativecommons.org/licenses/by/4.0 2019-06-27 2019-06-27 41 e103419 e103419 10.29374/2527-2179.bjvm103419 Serological evidence of canine arthropod-borne infections in an ecotone area of a natural reserve at the Pantanal, Brazil http://rbmv.org/index.php/BJVM/article/view/1037 <p>Arthropod-borne infections are dependent on environmental conditions; therefore, anthropomorphic meddling may disrupt the natural balance that maintains wildlife. It is common to find dogs roaming in Brazilian natural reserves, what favors the spillover of pathogens among species. The aim of this study was to determine the canine seroprevalence of <em>Ehrlichia canis</em>, <em>E. ewingii, Anaplasma platys, A. phagocytophilum, </em><em>Borrelia burgdorferi, Leishmania infantum</em> and <em>Dirofilaria immitis</em> using 84 serum samples from dogs from the border area of the SESC-Pantanal reserve (RPPN SESC-Pantanal 16°40 ́51 ́ ́S;56o17 ́45 ́ ́W) stored at the Laboratório de Protozoologia e Imunomodulação, Instituto Oswaldo Cruz. Samples were tested with SNAP Canine Leishmania Antibody Test (IDEXX Laboratories) or DPP canine visceral leishmaniasis test (Bio-Manguinhos) for the presence of <em>L. infantum</em> antibodies and with SNAP 4Dx Plus Test (IDEXX Laboratories) for <em>D. immitis, Ehrlichia spp., Anaplasma spp.</em> and <em>B. burgdorferi</em> seroprevalences.<br>The seroprevalence for tick-borne parasites was 79.8%, 13.1% for <em>L. infantum</em> and 7.1% for <em>D. immitis</em>. Since tick-borne parasites were the most frequent among the examined dogs it may be suggested that these parasites, ticks and hosts display special resilience skills to overcome the hostile local conditions. The low <em>L. infantum</em> prevalence suggests that the local biodiversity, especially the bird abundance, depurates Leishmania circulation. The mosquito-borne <em>D. immitis</em> prevalence was higher than expected, suggesting that the local flooding regime provides suitable mosquito-breeding spots. On the other hand, the only known vector in the area feeds on birds, therefore reducing mosquitoes worm burden and imparing <em>D. immitis</em> transmission.</p> Bruno Alberigi Norma Labarthe Flavia Cardoso Caroline Cunha Caroline Almeida Celeste Souza Flavya Mendes-de-Almeida Copyright (c) 2019 Bruno Alberigi, Norma Labarthe, Flavia Cardoso, Caroline Cunha, Caroline Almeida, Celeste Souza, Flavya Mendes-de-Almeida http://creativecommons.org/licenses/by/4.0 2019-08-19 2019-08-19 41 e103719 e103719 10.29374/2527-2179.bjvm103719 In situ histopathological and immunohistochemical characterization of rabies in the brains of naturally infected equines and bovines http://rbmv.org/index.php/BJVM/article/view/1014 <p>The present study sought to characterize the phenomena involved in the histopathology of rabies and to assess the presence and amount of viral antigen in situ in different brain regions of naturally infected equines and bovines. The histopathological examination showed several changes due to inflammation, being most often infected cells neurons. The neuronal degeneration involved 100% of cases, in addition to a diffuse lymphocytic Infiltration and gliosis, characterized by vasculitis and perivasculitis. The presence of Negri bodies was in most cases in discreet, and the fragments with higher concentrations of antigen by both techniques employed were the cerebellum and the brain stem. Immunohistochemistry test (IHC) demonstrated greater sensitivity when applied to samples of bovines. Our results showed that in 37.5% of the total number of fragments analyzed, viral inclusions were not observed, however, there was an inflammatory process. In relation to the species, the fragments from bovine’s animals showed a slight increase when examined under this feature. These findings highlight the importance of submitting samples from suspected animals for laboratory diagnostic, even when there are no apparent abnormal histological findings.&nbsp;</p> Samira Maria Achkar Elaine Raniero Fernandes Fernanda Guedes Enio Mori Carla Isabel Macedo Maria Irma e Seixas Duarte Copyright (c) 2019 Samira Maria Achkar, Elaine Raniero Fernandes, Fernanda Guedes, Enio Mori, Carla Isabel Macedo, Maria Irma e Seixas Duarte http://creativecommons.org/licenses/by/4.0 2019-09-27 2019-09-27 41 e101419 e101419 10.29374/2527-2179.bjvm101419 Diagnostic of gastrointestinal helminthiasis in canine feces and soils of public locations in Alegre city, Espírito Santo - Brazil http://rbmv.org/index.php/BJVM/article/view/1046 <p>Este trabalho teve como objetivo diagnosticar helmintoses gastrintestinais em fezes caninas e areias de locais públicos no município de Alegre-ES. Foram coletadas amostras de fezes e areias em locais públicos como praças, canteiros, calçadas e essas foram encaminhadas para o laboratório de Parasitologia do Hospital Veterinário do Centro de Ciências Agrárias e Engenharias da Universidade Federal do Espírito Santo, onde as amostras de fezes foram analisadas pela técnica de Willis-Mollay e Centrífugo-Flutuação Simples e as de areia analisadas pela técnica de Baermann modificada e pela técnica de Silva e colaboradores. Foram analisadas 92 amostras de fezes eforam positivas &nbsp;52% para <em>Ancylostoma </em>spp., 24% <em>Toxocara canis</em>, 8,7% <em>Dipylidium caninum</em>, 8,7% <em>Strongyloides</em> spp. e&nbsp; 1% <em>Taenia</em> sp. Nas amostras de solo e areia as larvas de ancilostomatídeos foram encontradas em 33,3%. Foi observado ainda que a técnica de Centrífugo-Flutuação Simples foi mais sensível para a detecção das formas parasitárias nas fezes de cães e que a técnica de Baermann foi mais sensível no diagnóstico das larvas encontradas na areia.</p> Maria Larissa Bitencourt Vidal Juliana Costa de Azevedo Marcelle Temporim de Moraes Isabella Vilhena Freire Martins Copyright (c) 2019 Maria Larissa Bitencourt Vidal, Juliana Costa de Azevedo, Marcelle Temporim de Moraes, Isabella Vilhena Freire Martins http://creativecommons.org/licenses/by/4.0 2019-09-27 2019-09-27 41 e104619 e104619 10.29374/10.29374/2527-2179.bjvm104619 Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato http://rbmv.org/index.php/BJVM/article/view/1068 <p><em>Trypanosoma rhipicephalis</em> Marotta et al. (2018) is a species isolated from <em>Rhipicephalus microplus</em> ticks collected from native bovine Seropédica, in the state of Rio de Janeiro. This study aimed to investigate the interaction of the tick <em>Rhipicephalus sanguineus</em> <em>s. l</em>. with <em>T. rhipicephalis</em> by in vitro artificial feeding. Eight females of <em>R. sanguineus</em> <em>s. l</em>. partially fed on rabbits. Tick infection was performed by an artificial feeding system using plastic tips for 12 hours. Canine blood used for feeding the ticks test group was previously infected with <em>T. rhipicephalis</em> 10<sup>8</sup>/mL. The hemolymph smear test was performed in all females after experimental infection. The daily posture was collected and organized in pools of each female per posture day. The eggs were divided into three groups, the first group for eggs PCR, the second for hatching and larval PCR, and the third group of eggs for isolation in cell culture. The evaluation of the presence of DNA in the macerated eggs of experimentally infected ticks showed two positive PCR samples. The evaluation of the presence of DNA in experimentally infected females showed all samples tested positive. For the hemolymph test, tick number 7 presented epimastigote developmental forms and amastigotes of <em>T. rhipicephalis</em>. Experimental infection by artificial feeding proved to be a suitable tool to study the interaction of <em>T. rhipicephalis</em> in <em>R. sanguineus</em> <em>s. l</em>. ticks. The results show the transovarial transmission of <em>T. rhipicephalis</em> by <em>R. sanguineus s. l</em>., as well as the interaction of the protozoan in the organism of this tick species.</p> Adivaldo Henrique da Fonseca Yasmim Kaulich Carolina Marotta Ribeiro Jaqueline Rodrigues de Almeida Valim Juliana Ferreira dos Santos Thays Figueiroa dos Santos Cláudia Bezerra da Silva Copyright (c) 2019 Adivaldo Henrique da Fonseca, Yasmim Kaulich, Carolina Marotta Ribeiro, Jaqueline Rodrigues de Almeida Valim, Juliana Ferreira dos Santos, Thays Figueiroa dos Santos, Cláudia Bezerra da Silva http://creativecommons.org/licenses/by/4.0 2019-09-30 2019-09-30 41 e104619 e104619 10.29374/bjvm106819 Colissepticemia neonatal em um canino – RELATO DE CASO http://rbmv.org/index.php/BJVM/article/view/970 <p><em>Escherichia coli</em> is a Gram-negative bacterium, belonging to commensal microbiota of the gastrointestinal tract of animals and humans. Some strains have virulence factors, which may cause enteritis and / or septicemia. In the septicemic form of the disease, the main predisposing factor is not ingestion or ingestion of insufficient amounts of colostrum. The objective of this study is to report a case of neonatal colissepticemia in a five-day-old canine. At necropsy of the corpse, congestion was observed in organs of the abdominal and thoracic cavities, and multifocal whitish areas with coalescents in the kidneys and lungs. Histopathological examination of the kidney, liver, lung and heart showed multifocal areas of necrosis with presence of myriad intralesional bacteria. In the organ samples sent to the microbiological diagnosis there was growth of <em>E. coli</em>. The clinical history, as well as the histopathological and microbiological findings allow us to infer that the cause of death was due to a sepsis caused by <em>E. coli</em>.</p> Taina dos Santos Alberti Josiane Bonel Fabiano da Rosa Venancio Cristiano Silva da Rosa Silvia Leal Ladeira Daniela Isabel Brayer Pereira Copyright (c) 2019 Taina dos Santos Alberti https://creativecommons.org/licenses/by/4.0 2019-03-15 2019-03-15 41 e97019 e97019 Pulmonary stenosis in a dog submitted to valvuloplasty http://rbmv.org/index.php/BJVM/article/view/1030 <p>The Congenital heart diseases are characterized by morphological defects in the embryonic development and the most frequent in dogs are: pulmonary and aortic valve stenosis, ventricular septal defect and persistente ductus arteriosus.&nbsp; They may occur asymptomatically or even evolve to congestive heart failure. The definitive diagnosis is made through the visualization of the alterations in the echocardiogram. In the present report, a French Bulldog, three month old, asymptomatic was presented to a private Veterinary practice in Rio de Janeiro, Brazil. Cardiac auscultation evidenced a systolic heart murmur in tricuspid focus and diastolic murmur in pulmonary focus. To better evaluate the dog &nbsp;chest radiography, electrocardiogram and echocardiogram were perfomed and &nbsp;allowed the diagnosis of pulmonary stenosis. The clinical treatment with beta-blocker was installed and the surgical procedure was performed with the ballon valvuloplasty. The aim of this study was to report a case of pulmonary artery stenosis in a puppy, treated clinically and surgically, emphasizing the importance of the physical and complementary cardiological exams.</p> Bruno Alberigi Nathália Marques de Oliveira Lemos Nicole Vallejo Correa dos Santos Julielton de Souza Barata Alexandre José Rodrigues Bendas Copyright (c) 2019 Bruno Alberigi, Nathália Marques de Oliveira Lemos, Nicole Vallejo Correa dos Santos, Julielton de Souza Barata, Alexandre José Rodrigues Bendas https://creativecommons.org/licenses/by/4.0 2019-05-03 2019-05-03 41 e103019 e103019 10.29374/2527-2179.bjvm103019 An autochthonous case of visceral canine leishmaniasis in Barra do Piraí-RJ http://rbmv.org/index.php/BJVM/article/view/990 <p>Visceral Leishmaniasis, a disease caused by the protozoan <em>Leishmania chagasi</em>, is a re-emergent anthropozoonosis, considered a major public health problem. In the urban area, the dog (<em>Canis familiaris</em>) is the main source of infection and canine enzootia has preceded the occurrence of human cases, in which infection in dogs has been more prevalent than in humans. In this context, the present study reports the occurrence of an autochthonous case of Visceral Canine Leishmaniasis in Barra do Piraí, RJ. After verification of a clinic compatible with canine visceral leishmaniasis, several diagnostic exams were performed, such as cytological examination of the ear tip and lymph node puncture, rapid immunochromatographic test DPP® (Dual Path Platform), ELISA, RIFI and culture of material obtained from of skin biopsy, lymph node and bone marrow puncture. With the clinical, serological and parasitological confirmation of <em>Leishmania</em> spp. the dog’s owner chose to euthanize this animal and 3 months later, another positive animal residing in the same place. Based on this report, it is necessary to implement the entomological and epidemiological surveillance in the county of Barra do Piraí, in order to detect the presence of the vector and / or canine enzootia prior to the installation of the anthropozoonotic cycle, allowing to adopt actions to control the disease and the occurrence of the disease in humans.</p> Flavia Clare Goulart de Carvalho Raphaela Fernandes Coelho Gabriele Barbosa Brandão Artur Augusto Velho Mendes Júnior Bruna de Azevedo Baêta Copyright (c) 2019 Flavia Clare Goulart de Carvalho##common.commaListSeparator##Raphaela Fernandes Coelho##common.commaListSeparator##Gabriele Barbosa Brandão##common.commaListSeparator##Artur Augusto Velho Mendes Júnior##common.commaListSeparator##Bruna de Azevedo Baêta http://creativecommons.org/licenses/by/4.0 2019-06-27 2019-06-27 41 e096019 e096019 10.29374/2527-2179.bjvm099019 Conservative treatment of ventricular tumors secondary to mammary neoplasia in dog http://rbmv.org/index.php/BJVM/article/view/994 <p>Cardiac neoplasms are uncommon, but secondary or metastatic neoplasms are relatively frequent, with an incidence 60 times higher than the primary ones. A particularly high frequency of metastatic cardiac involvement has been observed in lung squamous cell carcinomas, urinary tract tumors, and melanomas. However, the incidence is low because of breast tumors in dogs. Cardiac tumors may be symptomatic or, more commonly, an incidental finding observed during the investigation of unrelated problems. The clinical presentation is associated with that of a disseminated neoplasm, and cardiac metastases are generally asymptomatic but should be considered in patients with a history of neoplasia who present with impaired conduction, breathlessness, cardiomegaly, or arrhythmias. The present report aimed to describe a clinical case of cardiac metastasis of breast neoplasia in a dog in which we focusedon conservative clinical treatment and the importance of investigating the incidental clinical findings.</p> Mário dos Santos Filho Daniel Carvalho Hainfellner Nathália Marques de Oliveira Lemos Mateus Fernando Senra de Almeida Diana do Amaral Mendonça Marcela Rodrigues Valério de Oliveira Folha Bruno Ricardo Soares Alberigi Julio Israel Fernandes Jonimar Pereira Paiva Copyright (c) 2019 Mário dos Santos Filho, Daniel Carvalho Hainfellner, Nathália Marques de Oliveira Lemos, Mateus Fernando Senra de Almeida, Diana do Amaral Mendonça, Marcela Rodrigues Valério de Oliveira Folha, Bruno Ricardo Soares Alberigi, Julio Israel Fernandes, Jonimar Pereira Paiva http://creativecommons.org/licenses/by/4.0 2019-08-09 2019-08-09 41 e099419 e099419 Dilated cardiomyopathy in spider monkey (Ateles chamek, Humboldt, 1812) http://rbmv.org/index.php/BJVM/article/view/982 <div>Non-human primates are routinely studied and managed in zoos, conservation breeding centers, and</div> <div>research centers, but there is currently limited information regarding diseases that can affect these</div> <div>animals. Dilated cardiomyopathy is one of the most common cardiovascular diseases in small animal</div> <div>clinical practice. However, there are few reports of this condition occurring in non-human primates. Here,</div> <div>in a spider monkey (Ateles chamek)&nbsp;housed in the Rio de Janeiro Zoo, we report the occurrence of dilated</div> <div>cardiomyopathy, its etiology, pathophysiology, clinical presentation, diagnosis through clinical examination,</div> <div>and the use of complementary exams (radiographic, electrocardiographic, and echocardiographic), as</div> <div>well as the protocol, used in the treatment. In this case, it is assumed that the occurrence of the disease</div> <div>was related to the peripartum period due to the hormonal and metabolic changes that occurred, and the</div> <div>physiological interactions of gestation and puerperium</div> Mário dos Santos Filho Heloisa Helena de Campos Coutinho Chaves Bárbara Souza Neil Magalhães Andressa Kagohara Fernando Troccoli Alessandro Galhões Daniel de Almeida Balthazar Jonimar Pereira Paiva Copyright (c) 2019 Mário dos Santos Filho, Heloisa Helena de Campos Coutinho Chaves, Bárbara Souza Neil Magalhães, Andressa Kagohara, Fernando Troccoli, Alessandro Galhões, Daniel de Almeida Balthazar, Jonimar Pereira Paiva http://creativecommons.org/licenses/by/4.0 2019-09-05 2019-09-05 41 e098219 e098219 10.29374/2527-2179.bjvm098219 Feline cardiac lymphoma: a case report http://rbmv.org/index.php/BJVM/article/view/1000 <p>Cancer is the main cause of death among pet animals. FeLV, the feline leukemia virus, increases the odds of domestic felines’ developing lymphoma or leukemia 62 fold. The cardiac lymphoma is a rare neoplasia and little is known about it in Veterinary Medicine. Therefore, it has been sought to report a case of cardiac lymphoma in a two-year-old, FeLV-positive feline patient, who presented dyspnea, lack of appetite, progressive loss of weight, and apathy. By means of supplementary examination, the presence of a mass attached to the heat would be verified, and lymphoma was diagnosed upon histopathological examination. It is thus concluded that this neoplasia was associated to the feline leukemia virus and that, in spite of the supplementary examinations’ having been utterly important for a correct diagnosis, the lack of an early definition aggravated the clinical picture of the patient and hindered the implementation of specific treatment.</p> Henrique Inhauser Riceti Magalhães Karen Gonçalves Mendonça Ygor Henrique de Paula Fabiano Braz Romão Jeferson Borges Barcelos Wanessa Ribeiro Gontijo Dobritz Vera Lucia Pichioni Thaisa Reis dos Santos Copyright (c) 2019 Henrique Inhauser Riceti Magalhães, Karen Gonçalves Mendonça, Ygor Henrique de Paula, Fabiano Braz Romão, Jeferson Borges Barcelos, Wanessa Ribeiro Gontijo Dobritz, Vera Lucia Pichioni, Thaisa Reis dos Santos http://creativecommons.org/licenses/by/4.0 2019-09-12 2019-09-12 41 e100019 e100019 10.29374/2527-2179.bjvm100019 Extrahepatic Cholangiocarcinoma in an adult Black Howler Monkey (Primates: Atelidae) http://rbmv.org/index.php/BJVM/article/view/1015 <p>Over the past years, the number of neoplasm cases reported in nonhuman primates have considerably increased, with hepatocellular tumors reported in several species. Here a case report of extrahepatic cholangiocarcinoma in an adult male Black Howler Monkey (<em>Alouatta caraya</em>) is presented. The animal, kept as pet, was admitted at Veterinary Hospital presented clinical signs of cholestasis. After unsuccessful drug treatment, the animal was submitted to a cholecystoduodenostomy. During the surgical procedure, the hepatobiliary system was meticulously observed. The liver showed no macroscopic alterations, however, there was a solid mass throughout the common bile duct. Biopsy specimens were collected from the bile duct mass, liver and gallbladder for histological and immunohistochemical evaluations. Histological examination of the liver showed diffuse accentuated vacuolization and necrosis, besides hemosiderin accumulation inside hepatocytes. In the portal region, there was an accentuated proliferation of biliary ducts, which presented replete with bilirubin. Discrete neutrophilic inflammatory infiltrate and fibrosis were also observed. The bile duct mass showed a cellular proliferation of epithelial origin, confirmed by immunohistochemical analysis. The arrangement, characteristics and growth pattern of these cells revealed a malignant neoplasm. Despite the success of the procedure, the animal died hours later. Cholangiocarcinomas generally present high mortality rates, since its late clinical presentation make the treatments ineffective due to its large extensions at the time of diagnosis. The owner did not allow the necropsy, therefore the presence of other neoplasms and important organ alterations, which may be aggravated the patient's clinical condition, were not confirmed.</p> Mariele de Santi CAROLINE DO COUTO GABRIEL LUIZ MONTANHIM PAOLA CASTRO MORAES PAULO HENRIQUE LEAL BERTOLO ROSEMERI DE OLIVEIRA VASCONCELOS ACÁCIA REBELLO COUTINHO KARIN WERTHER Copyright (c) 2019 Mariele de Santi, CAROLINE DO COUTO, GABRIEL LUIZ MONTANHIM, PAOLA CASTRO MORAES, PAULO HENRIQUE LEAL BERTOLO, ROSEMERI DE OLIVEIRA VASCONCELOS, ACÁCIA REBELLO COUTINHO, KARIN WERTHER http://creativecommons.org/licenses/by/4.0 2019-09-28 2019-09-28 41 e101519 e101519 10.29374/2527-2179.bjvm101519