Values of magnesium in the vitreous humor of bovine in the south of the state of Rio de Janeiro
Due to the high incidence of hypomagnesemia in some countries and the absence of studies in Brazil, we want to evaluate the concentrations of magnesium in the vitreous humor of slaughtered bovines in the state of Rio de Janeiro in early spring, aiming to investigate the occurrence of animals under conditions of clinical hypomagnesemia. A total of 116 eyes of adult bovines of different ages and breeds were evaluated. We extracted an eye of each animal to evaluate, immediately after the slaughter and were manipulated to obtain the vitreous humor, under appropriate temperatures conditions. The vitreous humor was collected by aspiration with a 27x7 mm needle, inserted in the lateral corner of the eye. Approximately 1.0 ml of liquid was centrifuged (3000rpm, 15 minutes), the supernatant aspirated with a disposable micropipette, packaged in eppendorfs tubs without preservatives was kept at -20 °C. Tests were performed to determine magnesium concentrations using commercial kits in spectrophotometer automatic digital. The mean of the values obtained (1.99±0.3 mg/dL) was normal or low according to the source consulted. In 66 animals (56,9%) the concentration of magnesium in the vitreous humor was lower, 2.02 mg/dL, indicative of hypomagnesemia, demonstrating a high probability condition of clinical hypomagnesemia (tetany) can develop in the conditions outlined in the present study. The possibility of making a post-mortem diagnosis through vitreous humor analysis is important for the prevention of these diseases and to search for curative measures in those risky areas.
Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC 4.0), allowing the sharing of work with acknowledgement of the authorship of the work and initial publication in this journal. They may take additional contracts separately, with acknowledgement of authorship and initial publication in this journal. They are authorized to publish and distribute their work online after publication by BJVM.
The AUTHOR is informed and agrees with the journal that, therefore, its article may be incorporated into existing databases and scientific information systems (indexers and current databases) or existing in the future (indexers and future databases), under the conditions defined by the latter at all times, which shall at least involve the possibility that the holders of these databases may perform the following actions on the article: Reproduce, transmit and distribute the article, in whole or in part in any form or means of electronic transmission existing or developed in the future, including electronic transmission for research, viewing and printing purposes; extract figures, tables, illustrations, and other graphic objects, and capture metadata, captions, and related articles for search, preview, and print purposes. Scan and/or store images and electronic article text.