In vitro virulence of yeasts isolated from the cervicovaginal region of bitches
The present study verified the species of yeast present in cervicovaginal mucosa of bitches and the in vitro production of virulence factors. A total of 40 female dogs were divided into not neutered and pregnant groups and samples were collected implementing cervical-vaginal lavage with sterile saline. Isolation was performed in Brain and Heart Infusion Agar, followed by morphological, biochemical and physiological evaluations for phenotypical identification of yeasts, and determination of phospholipase and protease production using specific mediums. Fungal growth was obtained in 33 animals (82.5%), totalizing 60 isolates. Candida spp was the most prevalent genus, with 21 isolates (35.0%), followed by 19 (31.66%) Malassezia spp, 13 (21.66%) Rhodotorula spp, and 7 (11.66%) Trichosporon spp. For the evaluation of protease production, 36.66% were negative, while 33.33% positive and 30.0% strong positive. For phospholipase production 45.0% were negative, 16.66% positive, and 28.33% strong positive. Most of the isolates showed some degree of virulence, considering the production of these two enzymes, and were previously described as infectious agents of the reproductive tract. A better understanding of the genital tract microbiota could potentially clarify some reproductive disorders in small animal practice.
Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC 4.0), allowing the sharing of work with acknowledgement of the authorship of the work and initial publication in this journal. They may take additional contracts separately, with acknowledgement of authorship and initial publication in this journal. They are authorized to publish and distribute their work online after publication by BJVM.
The AUTHOR is informed and agrees with the journal that, therefore, its article may be incorporated into existing databases and scientific information systems (indexers and current databases) or existing in the future (indexers and future databases), under the conditions defined by the latter at all times, which shall at least involve the possibility that the holders of these databases may perform the following actions on the article: Reproduce, transmit and distribute the article, in whole or in part in any form or means of electronic transmission existing or developed in the future, including electronic transmission for research, viewing and printing purposes; extract figures, tables, illustrations, and other graphic objects, and capture metadata, captions, and related articles for search, preview, and print purposes. Scan and/or store images and electronic article text.