In vitro virulence of yeasts isolated from the cervicovaginal region of bitches
The present study verified the species of yeast present in cervicovaginal mucosa of bitches and the in vitro production of virulence factors. A total of 40 female dogs were divided into not neutered and pregnant groups and samples were collected implementing cervical-vaginal lavage with sterile saline. Isolation was performed in Brain and Heart Infusion Agar, followed by morphological, biochemical and physiological evaluations for phenotypical identification of yeasts, and determination of phospholipase and protease production using specific mediums. Fungal growth was obtained in 33 animals (82.5%), totalizing 60 isolates. Candida spp was the most prevalent genus, with 21 isolates (35.0%), followed by 19 (31.66%) Malassezia spp, 13 (21.66%) Rhodotorula spp, and 7 (11.66%) Trichosporon spp. For the evaluation of protease production, 36.66% were negative, while 33.33% positive and 30.0% strong positive. For phospholipase production 45.0% were negative, 16.66% positive, and 28.33% strong positive. Most of the isolates showed some degree of virulence, considering the production of these two enzymes, and were previously described as infectious agents of the reproductive tract. A better understanding of the genital tract microbiota could potentially clarify some reproductive disorders in small animal practice.
This work is licensed under a Creative Commons Attribution 4.0 International License.
- Authors keep the copyright and grant the magazine the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC 4.0), allowing the sharing of working with recognition of authorship of the work and initial publication in this journal.
- Authors are allowed to assume additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (e.g. publish in institutional repository or book chapter), with recognition of authorship and initial publication in this journal.
- Authors are allowed and are encouraged to publish and distribute your work online (e.g. in institutional repositories or on your personal page) at any point after the editorial process.
- in addition, the author is informed and consent to the magazine, so your article can be incorporated into bases and existing scientific information systems (indexers and current databases) or to exist in the future (indexers and future databases), under the conditions laid down by the latter at all times, which will involve at least the possibility that the holders of these databases can perform the following actions on the article:
a. Reproduce, transmit and distribute the article, in whole or in part in any form or by means of electronic transmission existing or developed in the future, including the electronic transmission for research purposes, displaying and printing.
b. Reproduce and distribute, in whole or in part, the article in print.Ability to translate certain parts of the article.
c. Extract figures, tables, illustrations and other graphic objects and capture metadata, subtitles and related article for research purposes, viewing and printing.
d.Transmission, distribution and reproduction by agents or authorised by the owners of distribution databases.
e. Preparation of bibliographic citations, abstracts and indexes and references related to capture selected parts of the article.
f. Scan and/or store images and electronic article text.