Molecular detection of Ehrlichia canis and Babesia canis vogeli in Rhipicephalus sanguineus sensu lato ticks from Cuba

  • Maylin Gonzalez Navarrete
  • Matheus Dias Cordeiro
  • Claudia Bezerra da Silva
  • Marcus Sandes Pires
  • Carla Carolina Dias Uzedo Ribeiro
  • Alejandro Cabezas Cruz
  • Carlos Luiz Massard
  • Eugenio Roque López
  • Adivaldo Henrique da Fonseca
Keywords: Tick-borne diseases, brown tick, ehrlichiosis, babesiosis, Cuba

Abstract

ABSTRACT. Navarrete M.G., Cordeiro M.D., Silva C.B., Pires, M.S., Ribeiro C.C.D.U., Cabezas-Cruz A., Massard C.L., López E.R & Foseca A.H. Molecular detection of Ehrlichia canis and Babesia canis vogeli in Rhipicephalus sanguineus sensu lato ticks from Cuba. [Detecção molecular de Ehrlichia canis e Babesia canis vogeli em Rhipicephalus sanguineus sensu lato de carrapatos em Cuba.] Revista Brasileira de Medicina Veterinária, 38(supl. 3):63-67, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23897-970, Brasil. E-mail: adivaldofonseca@yahoo.com Ticks (Acari: Ixodidae) are of relevant medical and veterinary importance worldwide because of the range of pathogens they can transmit. A survey was carried out to identify Babesia spp. and Ehrlichia spp. in ticks obtained from dogs of Cuba. A total of 431 ticks were collected from 378 dogs and the specimens were properly identified according to a taxonomic key for the genera. All ticks were identified as belong to the R. sanguineus sensu lato (s. l.) species. Genomic DNA was extracted from ticks with protocol using phenol/chloroform. Ticks were organized in pools and the DNA extracted were tested by a nested polymerase chain reaction (nPCR) to amplify 398 base pairs (bp) from the 16S ribosomal DNA (rDNA) of E. canis, and to a PCR to amplify approximately 560 bp from 18S ribosomal DNA (rDNA). Of the 49 pools tested, 8.16% (n=4/49) were positive for the E. canis by nPCR targeting the16S rDNA gene and only one pool (n=1/49; 2.04%) was positive for the gene 18S rDNA for Babesia canis. The four sequences obtained for the 16S rDNA fragment were identical to each other and resulted in 100% identity with E. canis from different countries. The obtained sequence of the 18S rDNA gene for Babesia spp. presented similarity of 100% with Babesia canis vogeli when compared to sequences deposited in Genbank. This is the first molecular detection of these agents in the tick R. sanguineus s. l. in Cuba.

Published
2016-12-23
How to Cite
Navarrete, M. G., Cordeiro, M. D., da Silva, C. B., Pires, M. S., Ribeiro, C. C. D. U., Cruz, A. C., Massard, C. L., López, E. R., & da Fonseca, A. H. (2016). Molecular detection of Ehrlichia canis and Babesia canis vogeli in Rhipicephalus sanguineus sensu lato ticks from Cuba. Brazilian Journal of Veterinary Medicine, 38(Supl. 3), 63-67. Retrieved from http://rbmv.org/index.php/BJVM/article/view/881

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