Campylobacter spp. isolates of swine feces submitted to transport stress: species and antimicrobial resistance

  • Roberta Torres Melo Núcleo de Saúde Pública, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil http://orcid.org/0000-0001-8265-5163
  • Mônica Ribeiro Gabriel Laboratório de Epidemiologia Molecular, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0003-1442-0834
  • Eliane Pereira Mendonça Laboratório de Biologia Molecular, Faculdade de Medicina Veterinária, Universidade de Uberaba – UNIUBE, Uberaba, MG, Brasil https://orcid.org/0000-0001-9754-4714
  • Jéssica Laura Miranda Peixoto Laboratório de Epidemiologia Molecular, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0002-9999-8223
  • Guilherme Paz Monteiro Laboratório de Epidemiologia Molecular, Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0002-7061-7044
  • Phelipe Augusto Borba Martins Peres Laboratório de Epidemiologia Molecular, Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0001-6796-2632
  • Edson Campos Valadares Junior Laboratório de Epidemiologia Molecular, Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0002-6715-4341
  • Denise Aparecida Rossi Laboratório de Epidemiologia Molecular, Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal de Uberlândia – UFU, Uberlândia, MG, Brasil https://orcid.org/0000-0001-5177-7467
Keywords: antibiotics, campylobacteriosis, feces, swine breeding.

Abstract

The influence of transport stress in the presence of Campylobacter spp. and the antimicrobial resistance profile were evaluated in feces of 60 pigs. The samples were collected at the finishing farm and after transport to the slaughterhouse, totaling 120 samples. Isolation was performed by plaque culture and identification of the species was obtained by biochemical tests confirmed with the PCR technique. Campylobacter spp. was isolated in 63.3% of the collected samples at the farm and 91.6% at the slaughterhouse, evidencing the influence of transport stress on the increase of the isolates (P<0.05). The species C. coli biotype I, C. jejuni biotype I and C. jejuni subsp. doylei were identified, with C. coli being more prevalent on the farm and C. jejuni in the slaughterhouse. Bacterial resistance was observed for all six classes of antibiotics tested.  Among them, the isolates presented greater resistance to lincomycin, tetracycline and nalidixic acid (98.9%), and greater sensitivity to amoxicillin (22.5%). The strains of C. coli showed higher antimicrobial resistance than those of C. jejuni (P<0.05). The results of this study point to the high isolation rates of C. coli and C. jejuni in pig feces destinated for slaughter and possible risks related to meat  consumption.  The high standards of resistance address the risk to public health.  

Published
2019-04-09
How to Cite
Melo, R. T., Gabriel, M. R., Mendonça, E. P., Peixoto, J. L. M., Monteiro, G. P., Peres, P. A. B. M., Valadares Junior, E. C., & Rossi, D. A. (2019). Campylobacter spp. isolates of swine feces submitted to transport stress: species and antimicrobial resistance. Brazilian Journal of Veterinary Medicine, 41, e086519. https://doi.org/10.29374/2527-2179.bjvm086519
Section
Scientific articles