METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
ABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: email@example.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC 4.0), allowing the sharing of work with acknowledgement of the authorship of the work and initial publication in this journal. They may take additional contracts separately, with acknowledgement of authorship and initial publication in this journal. They are authorized to publish and distribute their work online after publication by BJVM.
The AUTHOR is informed and agrees with the journal that, therefore, its article may be incorporated into existing databases and scientific information systems (indexers and current databases) or existing in the future (indexers and future databases), under the conditions defined by the latter at all times, which shall at least involve the possibility that the holders of these databases may perform the following actions on the article: Reproduce, transmit and distribute the article, in whole or in part in any form or means of electronic transmission existing or developed in the future, including electronic transmission for research, viewing and printing purposes; extract figures, tables, illustrations, and other graphic objects, and capture metadata, captions, and related articles for search, preview, and print purposes. Scan and/or store images and electronic article text.