MOLECULAR DIAGNOSIS OF LEPTOSPIROSIS IN BLOOD OF DOGS NATURALLY INFECTED
ABSTRACT. Meira C.D., Wenceslau A.A., Carvalho F.S., Albuquerque, G.R. & Dias R.C. Molecular diagnosis of Leptospirosis in blood of dogs naturally infected. [Diagnóstico molecular de leptospirose no sangue de cães naturalmente infectados]. Revista Brasileira de Medicina Veterinária, 33(1):7-11, 2011. Programa de Pós-Graduação em Ciência Animal, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km 16, Ilheús, BA 45662-900, Brasil. E-mail: firstname.lastname@example.org Leptospirosis is an acute and systemic infectious disease, which affects men and animals and is caused by pathogenic spirochetes of the genus Leptospira spp. The early diagnosis is important for the adoption of the appropriate therapy and prevention against the installation of critical symptomatologic forms. The Polymerase Chain Reaction (PCR) is being used for the diagnosis of infectious diseases showing efficiency for the diagnosis of the disease before the development of antibodies. The aim of this work was to evaluate the PCR technique with the primers G1 and G2 that amplify a fragment of 285 base pairs, specific of the region of gene secY of Leptospira spp, on 200 blood samples from domestic and stray dogs and determination of the risk factors associated from the Municipality of Ilhéus in the State of Bahia. Nine animals (4.5%) presented positive results for leptospirosis. There was no statistically significant difference between the prevalence of leptospirosis in domiciled or stray dogs (p= 1.0), sex (p= 0.455), age (p=0.644), access to the street (p= 0.113) and presence and/or contact with rodents (p= 0.362).