Evaluation of a single serologic test in screening of crossbred dairy herd exposed to Neospora caninum
ABSTRACT. de Magalhães, V.C.S., Costa, S.C.L., de Oliveira, U.V., de Almeida, C.P. & Munhoz, A.D.[Evaluation of a single serologic test in screening of crossbred dairy herd exposed to Neospora caninum]. Avaliação de um único exame sorológico na triagem de bovinos mestiços expostos à Neospora caninum. Revista Brasileira de Medicina Veterinária, 34(3):247-250, 2012. Laboratório de Análises Clínicas Veterinárias, Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45662-000, Brasil. Email: firstname.lastname@example.org. Crossbred animals from a dairy property with history of exposure to Neospora caninum were investigated in a longitudinal study. This study aimed to determine the relative specificity and sensitivity, positive and negative predictive values and agreement on a single sample detection of exposed and non-exposed animals to N. caninum. Blood was collected from 196 animals on three different occasions with a mean interval of 210 days between collections. The samples were examined for N. caninum using indirect fluorescent antibody test (cutoff titer 1:200). The serological results of the first sample collection were compared with those of the three consecutive collections. Positive animals were those with two or more reactive samples while negative ones were those with two or more negative samples. The sensitivity and specificity was 90.5% and 99.4%, respectively. The positive predictive value was 97.4% while the negative predictive value was 97.5%. The value of kappa was 0.92. High agreement, sensitivity and specificity suggest that a crossbred animal can be reliably characterized as exposed or non-exposed based on a single sample using a cutoff of 1:200 in the test. It is thus a valid method to determine the prevalence of N. caninum in mixed-race herds.
Authors retain the copyright and grant the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC 4.0), allowing the sharing of work with acknowledgement of the authorship of the work and initial publication in this journal. They may take additional contracts separately, with acknowledgement of authorship and initial publication in this journal. They are authorized to publish and distribute their work online after publication by BJVM.
The AUTHOR is informed and agrees with the journal that, therefore, its article may be incorporated into existing databases and scientific information systems (indexers and current databases) or existing in the future (indexers and future databases), under the conditions defined by the latter at all times, which shall at least involve the possibility that the holders of these databases may perform the following actions on the article: Reproduce, transmit and distribute the article, in whole or in part in any form or means of electronic transmission existing or developed in the future, including electronic transmission for research, viewing and printing purposes; extract figures, tables, illustrations, and other graphic objects, and capture metadata, captions, and related articles for search, preview, and print purposes. Scan and/or store images and electronic article text.