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ABSTRACT. Santos A.V., de Oliveira, R.A. & Albuquerque G.R. [The in vitro effect of neem extract (Azadirachta indica) and clove essential oil (Syzygium aromaticum) in the Rhipicephalus (Boophilus) microplus]. Efeito in vitro do extrato de nim (Azadirachta indica) e óleo essencial de cravo (Syzygium aromaticum) sobre Rhipicephalus (Boophilus) microplus. Revista Brasileira de Medicina Veterinária, 34(2):111-115, 2012. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, BA 415 KM 16, Salobrinho, Ilhéus, BA 45662-000, Brasil. E-mail: email@example.com. This study aimed to evaluate the in vitro effect of neem extract (Azadirachta indica) and clove essential oil (Syzygium aromaticum) on the tick Rhipicephalus (Boophilus) microplus. Extracts from the fresh leaves of the neem plant were obtained using water and ethanol solvents, while clove essential oil was obtained from the fruits of the Clove tree by hydrodistillation. The concentrations of aqueous and ethanoic neem extracts used were 10% and 50%, while of clove essential oil was used as 2.5% and 5%.These experiments were carried out on engorged females tick with in vitro immersion test. The average effectiveness of the extracts of neem was 15.04% until 74,39% depending de concentration and the solvent The average effectiveness of the 2.5% and 5% clove essential oil was 97.15 and 99.4%, respectively.