Rosmarinus officinalis (Lamiaceae): activity in vitro against ectoparasites of veterinary importance

  • Lilian Cristina de Sousa Oliveira Batista
  • Cristiane Nunes Coelho
  • Amanda Ferreira da Silva
  • Yara Peluso Cid
  • Viviane de Sousa Magalhães
  • Douglas Siqueira de Almeida Chaves
  • Katherina Coumendouros
Keywords: In vitro test, alecrim, ectoparasites, phenolics, pharmacopeia


ABSTRACT. Batista L.C.S.O., Coelho C.N., Silva A.F., Cid Y.P., Magalhães V.S., Chaves D.S.A. & Coumendouros K. [Rosmarinus officinalis (Lamiaceae): activity in vitro against ectoparasites of veterinary importance.] Rosmarinus officinalis (Lamiaceae): atividade in vitro frente a ectoparasitos de importância veterinária. Revista Brasileira de Medicina Veterinária, 35(Supl.2):119-125, 2013. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Anexo 1, Universidade Federal Rural do Rio de Janeiro, Campus Seropédica, Ecologia, BR 465 Km 7, Seropédica, RJ 23897-970, Brasil. E-mail: The aim of this work was evaluate the activity of Rosmarinus officinalis L. against the ectoparasites Ctenocephalides felis felis, Rhipicephalus sanguineus and Rhipicephalus microplus. The in vitro assays was realized according Drummond et al. (1973) using engorged females opposite of R. microplus and R. sanguineus, and impregnation filter paper test for C. felis felis. The crude extract of R. officinalis was obtained by maceration and their chemical composition was assessed by pharmacognostical analysis. The phytochemical analysis showed the presence of flavonoids, tannins, terpenes and saponins. Dilutions of the crude extract were prepared in several concentrations (range between 78 and 40,000 ppm). The results showed that the crude extract of R. officinalis was activity with dose-dependent effect in vitro for C. f. felis with efficacy of 95%. The efficacy for R. microplus was 28.50% and 45.33% for R. sanguineus.

How to Cite
Batista, L. C. de S. O., Coelho, C. N., da Silva, A. F., Cid, Y. P., Magalhães, V. de S., Chaves, D. S. de A., & Coumendouros, K. (2014). Rosmarinus officinalis (Lamiaceae): activity in vitro against ectoparasites of veterinary importance. Brazilian Journal of Veterinary Medicine, 35(Supl.2), 119-125. Retrieved from

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