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ABSTRACT. Ferreira G.S., Luz M.J., Atallah F.A., Peters J., Amaral L.G., Estupnañ O.F.T., Antunes F. & Oliveira A.L.A. [Tubal occlusion in dogs using the Technique Laparo-Endoscopic Single Site Surgery (Less) e video-Assisted technique]. Oclusão tubária em cães utilizando a técnica de Laparo-Endoscópica Single Site Surgery (Less) e a técnica videoassistida. Revista Brasileira de Medicina Veterinária, 35(Supl. 1):49-54, 2013. Curso de Pós-Graduação em Ciência Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, RJ 28013-602, Brasil. E-mail:email@example.com Sterilization techniques in female contraceptive methods represent important to prevent the occurrence of reproduction and diseases of the reproductive tract in the species. The tubal occlusion is a technique used in veterinary medicine, is considered a less invasive procedure than other sterilization techniques, performing this procedure using Minimally Invasive Surgical Techniques (TCMI) is spreading, and the subject of studies by various authors. The aim of this work was the realization of tubal occlusion in dogs through techniques of laparoscopic access single and two portals. Operated 13 bitches were randomly divided into two groups G1 (single access) and G2 (two gates). Surgical time was measured and divided into T1 (surgical access time) and T2 (time operation procedure). G1 to an incision was made caudal to the umbilicus, creating the portals from this and access to the abdominal cavity to perform the tubal occlusion using electrosurgery. For G2 was first created a portal umbilical laparoscope for introduction of the second portal was created so assisted. After access to the abdominal cavity was performed tubal occlusion using electrosurgery. We used the Mann-Whitney test for evaluation of surgical time, and Pearson correlation for variables surgical time and weight of the animal. The mean surgical times for the G1 were 5.3 ± 1.251 min for the completion of surgical access and 15.16 ± 2.699 min for the procedure. With respect to Group 2, the average access time of surgery was 10.14 ± 2.604 min and the mean procedure 7 ± 1.024 min. There urinary bladder perforation after insertion of the first trocar in a G1 animal, and the occurrence of burns due to the use of electrocautery in 3 animals of the same group. It can be concluded from this study that the use of laparoscopic techniques for single access and two portals to perform tubal occlusion in dogs are viable options for sterilization or population control of domestic dogs, since it is a technique for rapid implementation and minimally invasive. There were few complications related to the techniques evaluated.