Uterine inflammatory response in mares inseminated with fresh and frozen donkey semen
ABSTRACT. dos Santos C.S., de Jesus V.L.T., Pereira R. de C.G., Guedes Júnior D. da S., dos Santos M.A.J. & Jacob J.C.F. [Uterine inflammatory response in mares inseminated with fresh and frozen donkey semen]. Resposta inflamatória uterina em éguas inseminadas com sêmen fresco e congelado de jumento. Revista Brasileira de Medicina Veterinária, 35(2):155-162, 2013. Curso de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465 km 07, Seropédica, RJ 23890-000, Brasil. E-mail: email@example.com This study aimed to characterize the uterine inflammatory response of mares after insemination with fresh and frozen donkey semen. Seven mares were inseminated with fresh (G1) and frozen (G2) semen for two estrous cycles. The uterine inflammatory response was evaluated by ultrassonography and uterine cytology performed before (T1), four hours (T2) and 24 hours after insemination (T3). The results were analyzed by ANOVA Ultrassonography in T1 revealed no accumulation of intrauterine fluid, but in T2 and T3 there were accumulation of less than 2 cm in G1 and G2 (p> 0.05). Statistical analysis revealed significant differences between T1 in relation to other moments (T2 and T3), demonstrating that after insemination the uterine fluid accumulation observed for both groups was attributed to the inflammatory response to semen. Uterine cytology in T1 revealed mean percentage less than 5%, at T2 was higher than 50% (G1 and G2) and no significant difference (p> 0.05) between groups was observed. In G1 T3, there was a reduction in the percentage of neutrophils observed for T2 (p <0.001) for both groups showing that 24 hours after AI started the process of resolution of inflammation. The observed uterine inflammation in mares inseminated with fresh and frozen donkey semen was similar to those reported for equine semen in the same times of collection, and reduction of this response was also according to the literature. Therefore, the donkey’s sperm was able to promote uterine response similar to that described for equine sperm which was not related to the type of semen used, if fresh or cryopreserved.