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ABSTRACT. Furtado T.T., Flausino G., Leal P.D. de S., Ferreira J.P., McIntosh D., Flausino W., Teixeira Filho W.L., Paes-de-Almeida E.C. & Lopes C.W.G. [Diagnosis of cholangitis associate to mucocele in gallbladder due to Cyniclomyces guttulatus in dogs - Case reports.] Diagnóstico de colangite associado à mucocele da vesícula biliar por Cyniclomyces guttulatus em cães - Relato de casos. Revista Brasileira de Medicina Veterinária, 35(1):1-6, 2013. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: email@example.com Vegetative cells of an ascomycete, morphologically similar to previously descriptions of Cyniclomyces guttulatus were observed on the surface of the mucosa of the gallbladder, in samples of gastric lavage and feces of two dogs with a history characterized by diarrhea, vomiting, thickening of the stomach mucosa, and hypertrophy of the gallbladder. The diagnosis of biliary cholangitis was confirming by ultrasound examination and changes in liver enzymes. For treatment of this ascomycete fluconazol was used at a dose of 5mg/kg body weight which is efficient, because this was not observed ascomycete cells in gastric lavage and feces samples of these animals after treatment.