Prodduction of clone secretor of antibodies (IgG) againt of infection bursal disease virus
ABSTRACT. Marín S.Y., dos Santos B.M., Patarroyo J.H. & Vargas M.I. [Prodduction of clone secretor of antibodies (IgG) againt of infection bursal disease virus.] Produção de clones secretores de anticorpos (IgG) contra o vírus da doença infecciosa bursal. Revista Brasileira de Medicina Veterinária, 37(2):238-144, 2015. Programa de Pós-Graduação em Medicina Veterinária, Universidade Federal de Viçosa, Avenida PH Rolfs, s/n, Viçosa, MG 36570- 000, Brasil. E-mail: firstname.lastname@example.org Three clones secreting of antibodies (Abs) IgG against infection bursal disease virus IBDV was development. The IBDV was strain S706 (the intermediate vaccine) was replicated in VERO cell and purified by sucrose gradient, for ELISA and mice inoculation. For the immunization of the mice BALB/c using as a saponin adjuvant, that allowed an inflammation reaction which enhanced the antibody response, detectable by ELISA. The fusion of splenic cells of the immunized mice and the mieloma SP2/0 resulted in 2 hybridoma families (2H11 and 5C7). After cloning by limiting dilution, 3 clones secretors of Abs from IgG class were obtained. The 3 obtained Abs were capable to reveal the proteins turn VPX and VP2 by “western blotting”, respectively of 47 kDa and 41 kDa. The definition of the isotypes recognized by obtained Abs must be object of characterization to allow the use of the antibodies in immunodiagnostic tests such as immunofluorescence, immunocitochemistry or capture ELISA, for epidemiologic of the disease researches or to differentiate vaccine’s virus of the field virus.