Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro

Keywords: piroplasmosis, ticks, PCR, protozoon, molecular analysis.

Abstract

The canine babesiosis has a worldwide distribution, its one of the most important tick-borne disease. It is endemic in Brazil, where the prevalence is increasing in several áreas. Typically, anemia and thrombocytopenia are found in dogs with babesiosis, such as hematological changes. The aim of this study was to evaluate the piroplasms prevalence and hematologic changes in dogs at Small Animals Veterinary Hospital of Universidade Federal Rural do Rio de Janeiro (UFRRJ), through the amplification of the 18S rRNA gene using Polymerase Chain Reaction (PCR) and genetic description using sequencing. Blood samples of 250 dogs were randomly selected. From all samples, nine (3.6%) were positive for Babesia spp. using conventional PCR. The sequencing was performed on products from PCR and the positives samples were identified as Babesia vogeli. The main hematological alterations were anemia (66.7%) and thrombocytopenia (89%). The piroplasm that most affect dogs in the region is Babesia vogeli and it can develop anemia and thrombocytopenia.

References

Abdullahi, S. U., Mohammed, A. A., Trimnell, A. R., Sannusi, A., & Alafiatayo, R. (1990). Clinical and haematological findings in 70 naturally occurring cases of canine babesiosis. The Journal of Small Animal Practice, 31(3), 145147. http://dx.doi.org/10.1111/j.1748-5827.1990.tb00750.x.

Araújo, A. C., Silveira, J. A. G., Azevedo, S. S., Nieri-Bastos, F. A., Ribeiro, M. F. B., Labruna, M. B., & Horta, M. C. (2015). Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil. Pesquisa Veterinária Brasileira, 35(5), 456-461. http://dx.doi.org/10.1590/S0100-736X2015000500012.

Ayoob, A. L., Hackner, S. G., & Prittie, J. (2010). Clinical management of canine babesiosis. Journal of Veterinary Emergency and Critical Care (San Antonio, Tex.: 2001), 20(1), 77-89. http://dx.doi.org/10.1111/j.1476-4431.2009.00489.x. PMid:20230437.

Bastos, C. V., Moreira, S. M., & Passos, L. M. F. (2004). A retrospective study (1998-2001) on canine babesiosis in Belo Horizonte, Minas Gerais State, Brazil. Annals of the New York Academy of Sciences, 1026(1), 158-160. http:// dx.doi.org/10.1196/annals.1307.023. PMid:15604486.

Beck, R., Vojta, L., Mrljak, V., Marinculic, A., Beck, A., Zivicnjak, T., & Cacciò, S. M. (2009). Diversity of Babesia and Theileria species in symptomatic and asymptomatic dogs in Croatia. International Journal for Parasitology, 39(7), 843-848. http://dx.doi.org/10.1016/j.ijpara.2008.12.005. PMid:19367832.

Birkenheuer, A. J. (2012). Babesiosis. In C. E. Greene (Ed.), Infectious diseases of the dog and cat (4th ed., pp. 771784). USA:Elsevier.

Birkenheuer, A. J., Neel, J., Ruslander, D., Levy, M. G., & Breitschwerdt, E. B. (2004). Detection and molecular characterization of a novel large Babesia species in a dog. Veterinary Parasitology, 124(3-4), 151-160. http:// dx.doi.org/10.1016/j.vetpar.2004.07.008. PMid:15381295.

Bourdoiseau, G. (2006). Canine babesiosis in France. Veterinary Parasitology, 138(1-2), 118-125. http://dx.doi. org/10.1016/j.vetpar.2006.01.046. PMid:16507334.

Cacciò, S. M., Antunovic, B., Moretti, A., Mangili, V., Marinculic, A., Baric, R. R., Slemenda, S. B., & Pieniazek, N. J. (2002). Molecular characterization of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs. Veterinary Parasitology, 106(4), 285-292. http://dx.doi.org/10.1016/S0304-4017(02)00112-7. PMid:12079734.

Carli, E., Tasca, S., Trotta, M., Furlanello, T., Caldin, M., & Solano-Gallego, L. (2009). Detection of erythrocyte binding IgM and IgG by flow cytometry in sick dogs with Babesia canis canis or Babesia canis vogeli infection. Veterinary Parasitology, 162(1-2), 51-57. http://dx.doi.org/10.1016/j.vetpar.2009.02.002. PMid:19269745.

Carret, C., Walas, F., Carcy, B., Grande, N., Précigout, E., Moubri, K., Schetters, T. P., & Gorenflot, A. (1999). Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. The Journal of Eukaryotic Microbiology, 46(3), 298-303. http://dx.doi.org/10.1111/j.1550-7408.1999.tb05128.x. PMid:10377990.

Criado-Fornelio, A., Martinez-Marcos, A., Buling-Saraña, A., & Barba-Carretero, J. C. (2003). Molecular studies on Babesia, Theileria and Hepatozoon in Southern Europe: Part I. Epizootiological aspects. Veterinary Parasitology, 113(3-4), 189-201. http://dx.doi.org/10.1016/S0304-4017(03)00078-5. PMid:12719133.

Eiras, D. F., Craviotto, M. B., Baneth, G., & Moré, G. (2014). First report of Rangelia vitalii infection (canine rangeliosis) in Argentina. Parasitology International, 63(5), 729-734. http://dx.doi.org/10.1016/j.parint.2014.06.003. PMid:24970768.

Felsenstein, J. PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, 2005.

Furlanello, T., Fiorio, F., Caldin, M., Lubas, G., & Solano-Gallego, L. (2005). Clinicalpathological findings in naturally occurring cases of babesiosis caused by large form Babesia from dogs of northeastern Italy. Veterinary Parasitology, 134(1-2), 77-85. http://dx.doi.org/10.1016/j.vetpar.2005.07.016. PMid:16112810.

García, A. T. C. (2006). Piroplasma infection in dogs in northern Spain. Veterinary Parasitology, 138(1-2), 97-102. http://dx.doi.org/10.1016/j.vetpar.2006.01.043. PMid:16513282.

Guimarães, J. C., Albernaz, A. P., Machado, J. A., Junior, O. A. M., & Garcia, L. N. N. (2004). Aspectos clinicolaboratoriais da babesiose canina na cidade de Campos dos Goytacazes, RJ. Revista Brasileira de Parasitologia Veterinária, 13, 229.

Irwin, P. J. (2009). Canine babesiosis: from molecular taxonomy to control. Parasites & Vectors, 2(Suppl 1), S4. http://dx.doi.org/10.1186/1756-3305-2-S1-S4. Kjemtrup, A. M., & Conrad, P. A. (2006). A review of the small canine piroplasms from California: Babesia conradae in the literature. Veterinary Parasitology, 138(1-2), 112-117. http://dx.doi.org/10.1016/j.vetpar.2006.01.045. PMid:16522352.

Kjemtrup, A. M., Wainwright, K., Miller, M., Penzhorn, B. L., & Carreno, R. A. (2006). Babesia conradae, sp. nov., a small canine Babesia identified in California. Veterinary Parasitology, 138(1-2), 103-111. http://dx.doi.org/10.1016/j. vetpar.2006.01.044. PMid:16524663.

Lack, J. B., Reichard, M. V., & Van Den Bussche, R. A. (2012). Phylogeny and evolution of the Piroplasmida as inferred from 18S rRNA sequences. Internaciol Journal for Parasitology, 42(4), 353-363. http://dx.doi.org/10.1016/j. ijpara.2012.02.005. PMid:22429769.

Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J., & Higgins, D. G. (2007).Clustal W and Clustal X version 2.2. Bioinformatics (Oxford, England), 23(21), 2947-2948. http://dx.doi.org/10.1093/bioinformatics/btm404. PMid:17846036.

Lehtinen, L. E., Birkenheuer, A. J., Droleskey, R. E., & Holman, P. J. (2008). In vitro cultivation of a newly recognized Babesia sp. in dogs in North Carolina. Veterinary Parasitology, 151(2-4), 150-157. http://dx.doi.org/10.1016/j. vetpar.2007.10.022. PMid:18083310.

Published
2020-10-07
How to Cite
Assad, R. Q., Junior, E. S., Lambert, M. M., Correa, C. B. S., Lemos, T. D., Toma, H. K., Scott, F. B., & Almosny, N. R. P. (2020). Molecular detection and hematological changes of dogs samples naturally infected by Babesia vogeli at metropolitan region of Rio de Janeiro. Brazilian Journal of Veterinary Medicine, 42(1), e032120. https://doi.org/10.29374/2527-2179.bjvm032120
Section
Scientific articles