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ABSTRACT. Pereira C.S., Santos L.M.M., Almeida J.F., Pereira V.L.A. & Nascimento E.R. [Mastitis Detected Throught Somatic Cell Count and Bacterial isolation in negative goats for Staphylococcus aureus.] Mastite por contagem de células somáticas e isolamento bacteriano em cabras negativas para Staphylococcus aureus. Revista Brasileira de Medicina Veterinária, 38(1):99-104, 2016. Programa de Pós-Graduação em Higiene Veterinária e Processamento Tecnológico de Produtos de Origem Animal, Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brasil Filho, 64, Vital Brazil, Niterói, RJ 24230-340, Brasil. E-mail: email@example.com A subclinical mastitis study was conducted in nine dairy goat herds in the Rio de Janeiro state to determine the occurrence of infection, detection of Staphylococcus aureus by polymerase chain reaction, evaluate microbiological and cellular profiles of the milk and associate the influence of somatic cell count (SCC) to bacterial isolation. A total of 133 raw milk sample were collected for microbiological culture, Staphylococcus aureus by polymerase chain reaction and SCC. There was bacterial growth in 64 samples (48%), among those 10 obtained isolation of two microorganisms associated. Seventy-seven isolates were found distributed in the following way: 45 (58,4%) coagulase-negative Staphylococcus, 9 (11,58%) coagulase-positive Staphylococcus e gram negative bacilli of the Enterobacteriaceae family, 5 (6,49%) Bacillus spp., 4 (5,19%) Streptococcus spp., 3 (3,89%) Enterococcus spp. e 1 (1,29%) E. coli and Serratia sp. The association of coagulase-negative Staphylococcus and Gram negative Bacilli had the largest number, with 50%, followed by 20% coagulase positive Staphylococcus + Enterococcus sp. and 10% coagulase-negative Staphylococcus + Bacillus spp., Streptococcus spp. + Bacillus spp. and Streptococcus spp. + Gram Negative Bacilli. No milk sample obtained positive for Staphylococcus aureus in culture and PCR. Mean SCC was 2.152.000 cells/ mL of milk. There was no significant association between bacterial isolation and the values obtained in somatic cell count (t-Student p > 0,05). Statistical difference was observed in SCC average of the properties studied (p <0,05). We conclude that Coagulase Negative Staphylococcus was isolated bacterial group with greater frequency and SCC does not correlate with the results of bacterial isolation and must be carefully evaluated in studies of subclinical mastitis in goats.