Difficulties in diagnosis of bovine tuberculosis in suggestive lesions

  • Marcio Junio Lima Siconelli Medical School of Ribeirão Preto (FMRP/USP)
  • David Attuy Vey da Silva Faculty of Veterinary Medicine and Zootecnia (FMVZ/USP)
  • Andressa de Souza Pollo Faculty of Agrarian and Veterinary Sciences (FCAV/Unesp)
  • Karina Paes Bürger Faculty of Agrarian and Veterinary Sciences (FCAV/Unesp)
Keywords: Cattle, PCR, M. bovis, zoonosis

Abstract

The bovine tuberculosis is a zoonotic disease with importance to the public health. The post mortem identification and its fast and accurate diagnosis combined with the epidemiology knowledge are essential to fight against the advance of this disease. Therefore, the present study aimed to perform the diagnosis of Mycobacterium bovis, by molecular detection and also by histopathologic lesion characterization and identification of acid-fast bacilli (AFB), seeking to compare them. During slaughter of cattle in slaughter plants, under Federal Inspection Service in São Paulo State, were collected and identified, in duplicate, tissue samples with suggestive lesions of tuberculosis. One of the samples was intended for histopathology, being performed the hematoxylin-eosin (HE) staining for lesion characterization and the Ziehl-Neelsen (ZN) staining to identification the AFB. The other sample was separated to the molecular diagnosis, Polymerase Chain Reaction (PCR) and nested PCR. The bacterial DNA present in the lesion was extracted and the PCR was performed, showing 20.84% (10/48) of positive samples. However, when the results were compared with more sensitive tests (nested PCR) and cheapest ones (histopathologycal characterization of lesions and the AFB identification), different results were found. When performing nested PCR in negative samples by conventional PCR (38/48), it was found more 18.74% (9/38) of positive samples resulting in 39.58% (19/48) of positive samples by molecular diagnosis from tissue lesions. However, histopathological characterization by hematoxylin-eosin resulted in 66.67% (32/48) of positive samples and the identification of AFB by Ziehl-Neelsen, 54.17% (26/48) of positive samples for bovine tuberculosis. The molecular tissue diagnosis from lesions is possible, but it should not be the only method used for bovine tuberculosis diagnosis.

Author Biographies

Marcio Junio Lima Siconelli, Medical School of Ribeirão Preto (FMRP/USP)

Veterinarian. Specialist in Preventive Veterinary Medicine and Public Health; Master's Degree in Basic and Applied Immunology, Department of Biochemistry and Immunology, Medical School of Ribeirão Preto (FMRP), USP, Av. Bandeirantes, 3900 - Monte Alegre - CEP: 14049-900 Ribeirão Preto, São Paulo, Brazil. + Author for correspondence.

David Attuy Vey da Silva, Faculty of Veterinary Medicine and Zootecnia (FMVZ/USP)

Veterinarian. MSc. Ph.D student in Experimental Epidemiology Applied to Zoonoses, Department of Preventive Veterinary Medicine and Animal Health - VPS / FMVZ / USP Câmpus Fernando Costa. Av. Duque de Caxias Norte, 225 - 13635-900, Pirassununga, São Paulo.

Andressa de Souza Pollo, Faculty of Agrarian and Veterinary Sciences (FCAV/Unesp)

Agronomic Engineer. Ph.D. Assistant of Academic Support IV, Laboratory of Molecular Epidemiology, Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agrarian and Veterinary Sciences (FCAV), Unesp, Path of Access Prof. Paulo Donato Castellane s / n -14884-900, Jaboticabal, São Paulo.

Karina Paes Bürger, Faculty of Agrarian and Veterinary Sciences (FCAV/Unesp)

Veterinarian. Ph.D. Professor of the Department of Preventive Veterinary Medicine and Animal Reproduction, Faculty of Agrarian and Veterinary Sciences (FCAV), Unesp, Path of Access Prof. Paulo Donato Castellane s / n -14884-900, Jaboticabal, São Paulo.. 

Published
2018-06-29
Section
Scientific articles