In vitro evaluation of the acaricidal activity of Syzygium aromaticum (L.) essential oil and eugenol against non-fed larvae of Rhipicephalus sanguineus

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Monique Moraes Lambert
Douglas Siqueira de Almeida Chaves
Barbara Rauta de Avelar
Diefrey Ribeiro Campos
Debora Azevedo Borges
Leandra Oliveira Moreira
Geraldo Augusto Pereira
Yara Peluso Cid
Fabio Barbour Scott
Katherina Coumendouros

Abstract

 Plant extracts and essential oils have been showing potential for use as acaricides. Rhipicephalus sanguineus, the brown tick, mainly parasitizes dogs and is responsible for pathogens transmission. The insecticidal and acaricidal activity of the essential oil (EO) of Syzygium aromaticum and eugenol have already been reported, however the activity against R. sanguineus is lacked. To evaluate the acaricidal activity of S. aromaticum EO and eugenol against larvae of R. sanguineus, in vitro assays were performed using the larval immersion test. Bioassays were performed in duplicate, within the concentration range of 0.078 to 40 mg.mL-1. Mortality assessment was performed after 24 hours. Mortality above 90% was achieved through using the EO and eugenol at concentrations greater than 5 and 10 mg.mL-1, respectively. The LC50 values of 1.4 and 3.3 mg.mL-1 for eugenol and S. aromaticum EO, respectively, while the LC90 values found for eugenol and S. aromaticum EO were, respectively, 6.4 and 6.7 mg.mL-1 were calculated. These compounds present activity against R. sanguineus larvae and demonstrate potential for use as acaricides.

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How to Cite
Moraes Lambert, M., Siqueira de Almeida Chaves, D. ., Rauta de Avelar, B. ., Ribeiro Campos, D. ., Azevedo Borges, D. ., Oliveira Moreira, L. ., Pereira, G. A. ., Peluso Cid, Y., Barbour Scott, F. ., & Coumendouros, K. (2021). In vitro evaluation of the acaricidal activity of Syzygium aromaticum (L.) essential oil and eugenol against non-fed larvae of Rhipicephalus sanguineus. Brazilian Journal of Veterinary Medicine, 43(1). https://doi.org/10.29374/2527-2179.bjvm002620
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Scientific articles

References

Araújo, L. X., Novato, T. P. L., Zeringota, V., Maturano, R., Melo, D., Silva, B. C., Daemon, E., Carvalho, M. G., & Monteiro, C. M. O. (2016). Synergism of thymol, carvacrol and eugenol in larvae of the cattle tick, Rhipicephalus microplus, and brown dog tick, Rhipicephalus sanguineus. Medical and Veterinary Entomology, 30(4), 377-382. http://dx.doi.org/10.1111/mve.12181. PMid:27392803.

Bagavan, A., Rahuman, A. A., Kamaraj, C., Elango, G., Zahir, A. A., Jayaseelan, C., Santhoshkumar, T., & Marimuthu, S. (2011). Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis (Phthiraptera: Pediculidae). Parasitology Research, 109(5), 1329-1340. http://dx.doi.org/10.1007/s00436-011-2425-1. PMid:21541752.

Barreto, C. F., Carvasin, G. M., Silva, H. H. G. D., & Silva, I. G. D. (2006). Estudo das alterações morfo-histológicas em larvas de Aedes aegypi (Diptera, Culicidae) submetidas ao extrato bruto etanólico de Sapindus saponaria Lin (Sapindaceae). Journal of Tropical Pathology, 35(1), 37-57. http://dx.doi.org/10.5216/rpt.v35i1.1891.

Chagas, A. C. S., Barros, L. D., Cotinguiba, F., Furlan, M., Giglioti, R., Sena Oliveira, M. C., & Bizzo, H. R. (2012). In vitro efficacy of plant extracts and synthesized substances on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Parasitology Research, 110(1), 295-303. http://dx.doi.org/10.1007/s00436-011-2488-z. PMid:21695568.

Chagas, A. C. S., Passos, W. M., Prates, H. T., Leite, R. C., Furlong, J., & Fortes, I. C. P. (2002). Efeito acaricida de óleos essenciais e concentrados emulsionáveis de Eucalyptus spp. em Boophilus microplus. Brazilian Journal of Veterinary Research and Animal Science, 39(5), 247-253. http://dx.doi.org/10.1590/S1413-95962002000500006.

Coelho, L., Paula, L. G. F., Alves, S. D. G. A., Sampaio, A. L. N., Bezerra, G. P., Vilela, F. M. P., Matos, R. D. S., Zeringóta, V., Borges, L. M. F., & Monteiro, C.. (2020). Combination of thymol and eugenol for the control of Rhipicephalus sanguineus sensu lato: evaluation of synergism on immature stages and formulation development. Veterinary Parasitology, 277, 108989. http://dx.doi.org/10.1016/j.vetpar.2019.108989. PMid:31794909.

Dantas-Torres, F. (2008). The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): From taxonomy to control. Veterinary Parasitology, 152(3-4), 173-185. http://dx.doi.org/10.1016/j.vetpar.2007.12.030. PMid:18280045.

Dantas-Torres, F., & Otranto, D. (2015). Further thoughts on the taxonomy and vector role of Rhipicephalus sanguineus group ticks. Veterinary Parasitology, 208(1-2), 9-13. http://dx.doi.org/10.1016/j.vetpar.2014.12.014. PMid:25579394.

Dantas-Torres, F. (2010). Biology and ecology of the brown dog tick, Rhipicephalus sanguineus. Parasites & Vectors, 3(1), 26-36. http://dx.doi.org/10.1186/1756-3305-3-26. PMid:20377860.

Eiden, A. L., Kaufman, P. E., Oi, F. M., Allan, S. A., & Miller, R. J.. (2015). Detection of permethrin resistance and fipronil tolerance in Rhipicephalus sanguineus (Acari: Ixodidae) in the United States. Journal of Medical Entomology, 52(3), 429-436. http://dx.doi.org/10.1093/jme/tjv005. PMid:26334817.

Ellse, L., & Wall, R. (2014). The use of essential oils in veterinary ectoparasite control: a review. Medical and Veterinary Entomology, 28(3), 233-243. http://dx.doi.org/10.1111/mve.12033. PMid:24147451.

Ferreira, F. M., Delmonte, C. C., Novato, T. L. P., Monteiro, C. M. O., Daemon, E., Vilela, F. M. P., & Amaral, M. P. H. (2018). Acaricidal activity of essential oil of Syzygium aromaticum, hydrolate and eugenol formulated or free on larvae and engorged females of Rhipicephalus microplus. Medical and Veterinary Entomology, 32(1), 41-47. http://dx.doi.org/10.1111/mve.12259. PMid:28833280.

Ferreira, T. P., Cid, Y. P., Cardilo, M. A., Santos, G. C. M., Avelar, B. R., & Freitas, J. P. (2019). In vitro Acaricidal Activity of Ocimum gratissimum Essential Oil on Rhipicephalus sanguineus, Amblyomma sculptum and Rhipicephalus microplus Larvae. Revista Virtual de Química., 11(5), 1604-1613. http://dx.doi.org/10.21577/1984-6835.20190112.

Jairoce, C. F., Teixeira, C. M., Nunes, C. F. P., Nunes, A. M., Pereira, C. M. P., & Garcia, F. R. M. (2016). Insecticide activity of clove essential oil on bean weevil and maize weevil. Revista Brasileira de Engenharia Agrícola e Ambiental, 20(1), 72-77. http://dx.doi.org/10.1590/1807-1929/agriambi.v20n1p72-77.

Lambert, M. M., Campos, D. R., Borges, D. A., Avelar, B. R., Ferreira, T. P., Cid, Y. P., & Coumendouros, K. (2020). Activity of Syzygium aromaticum essential oil and its main constituent eugenol in the inhibition of the development of Ctenocephalides felis felis and the control of adults. Veterinary Parasitology, 282(1), 109126. http://dx.doi.org/10.1016/j.vetpar.2020.109126. PMid:32417602.

Mbaveng, A. T., & Kuete, V. (2017). Syzygium aromaticum. In V. Kuete (Ed.), Medicinal spices and vegetables from Africa: Therapeutic potential against metabolic, inflammatory, infectious and systemic diseases (1st ed., pp. 611- 625). Amsterdam: Elsevier. http://dx.doi.org/10.1016/B978-0-12-809286-6.00029-7.

Monteiro, C. M., Maturano, R., Daemon, E., Catunda-Junior, F. E. A., Calmon, F., & Senra, T. S. (2012). Acaricidal activity of eugenol on Rhipicephalus microplus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Parasitology Research, 111(3), 11295-11300. http://dx.doi.org/10.1007/s00436-012-2964-0. PMid:22622689.

Novato, T., Gomes, G. A., Zeringóta, V., Franco, C. T., Oliveira, D. R., & Melo, D. (2018). In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae). Veterinary Parasitology, 260(1), 1-4. http://dx.doi.org/10.1016/j.vetpar.2018.07.009. PMid:30197007.

Rodriguez‐Vivas, R. I., Ojeda‐Chi, M. M., Trinidad‐Martinez, I., & Bolio‐González, M. E. (2017). First report of amitraz and cypermethrin resistance in Rhipicephalus sanguineus sensu lato infesting dogs in Mexico. Medical and Veterinary Entomology, 31(1), 72-77. http://dx.doi.org/10.1111/mve.12207. PMid:27859488.

Senra, T. O., Calmon, F., Zeringóta, V., Monteiro, C. M., Maturano, R., Matos, R. S., Melo, D., Gomes, G. A., Carvalho, M. G., & Daemon, E. (2013). Investigation of activity of monoterpenes and phenylpropanoids against immature stages of Amblyomma cajennense and Rhipicephalus sanguineus (Acari: Ixodidae). Parasitology Research, 112(10), 3471-3476. http://dx.doi.org/10.1007/s00436-013-3527-8. PMid:23842886.

Valente, P. P., Amorim, J. M., Castilho, R. O., Leite, R. C., & Ribeiro, M. F. B. (2014). In vitro acaricidal efficacy of plant extracts from Brazilian flora and isolated substances against Rhipicephalus microplus (Acari: Ixodidae). Parasitology Research, 113(1), 417-423. http://dx.doi.org/10.1007/s00436-013-3670-2. PMid:24221889.