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ABSTRACT. Oliveira B.R., Gundim L.F., Lima A.M.C. & Medeiros A.A. [Brachyspira hyodysenteriae and Brachyspira pilosicoli research in pigs with diarrhea in Espírito Santo, Brazil.] Investigação de Brachyspira hyodysenteriae e Brachyspira pilosicoli em suínos com diarreia no Espírito Santo, Brasil. Revista Brasileira de Medicina Veterinária, 38(3):287-291, 2016. Programa de Pós-Gradua- ção em Ciências Veterinárias, Universidade Federal de Uberlândia, Rua Ceará, s/n, Bloco 2D, Sala 3, Campus Umuarama, Uberlândia, MG 38400-902, Brasil. E-mail: email@example.com This study aimed investigate the presence of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pigs with clinical signs of diarrhea, by real time polymerase chain reaction - qPCR, and evaluate epidemiological conditions of the properties. Samples were obtained from 30 farms in 10 different Municipalities in the State of Espirito Santo (ES). With the aid of spatulas and rectal swabs collected up the feces and made pools of feces 3-5 animals. We apply an epidemiological survey on farms for identification of the risk factors.As samples were analyzed by qPCR (RT-PCR) in a commercial laboratory in triplicate for identification of B. hyodysenteriae and B. pilosicoli. No positive samples were detected for B. hyodysenteriae and B. pilosicoli. With regard to epidemiological data, 27 farms (90%) had veterinary medical assistance, two farms (6.67%) did not have complete cycle, 12 properties (40%) used for compost waste and 18 (60%) cesspools. Twenty-nine properties (96.7%) introduced animals from other properties to spare the squad. The use of antimicrobial and low density production units in the ES may have interfered in identifying the investigated etiologic agents.