Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato

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Adivaldo Henrique da Fonseca
https://orcid.org/0000-0002-5834-141X
Yasmim Kaulich
https://orcid.org/0000-0003-2691-6351
Carolina Marotta Ribeiro
https://orcid.org/0000-0002-5361-9136
Jaqueline Rodrigues de Almeida Valim
https://orcid.org/0000-0003-1173-8601
Juliana Ferreira dos Santos
https://orcid.org/0000-0002-6599-710X
Thays Figueiroa dos Santos
https://orcid.org/0000-0001-7222-2030
Cláudia Bezerra da Silva
https://orcid.org/0000-0003-1761-2110

Abstract

Trypanosoma rhipicephalis Marotta et al. (2018) is a species isolated from Rhipicephalus microplus ticks collected from native bovine Seropédica, in the state of Rio de Janeiro. This study aimed to investigate the interaction of the tick Rhipicephalus sanguineus s. l. with T. rhipicephalis by in vitro artificial feeding. Eight females of R. sanguineus s. l. partially fed on rabbits. Tick infection was performed by an artificial feeding system using plastic tips for 12 hours. Canine blood used for feeding the ticks test group was previously infected with T. rhipicephalis 108/mL. The hemolymph smear test was performed in all females after experimental infection. The daily posture was collected and organized in pools of each female per posture day. The eggs were divided into three groups, the first group for eggs PCR, the second for hatching and larval PCR, and the third group of eggs for isolation in cell culture. The evaluation of the presence of DNA in the macerated eggs of experimentally infected ticks showed two positive PCR samples. The evaluation of the presence of DNA in experimentally infected females showed all samples tested positive. For the hemolymph test, tick number 7 presented epimastigote developmental forms and amastigotes of T. rhipicephalis. Experimental infection by artificial feeding proved to be a suitable tool to study the interaction of T. rhipicephalis in R. sanguineus s. l. ticks. The results show the transovarial transmission of T. rhipicephalis by R. sanguineus s. l., as well as the interaction of the protozoan in the organism of this tick species.

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How to Cite
da Fonseca, A. H., Kaulich, Y., Ribeiro, C. M. ., Valim, J. R. de A., dos Santos, J. F., dos Santos, T. F. ., & da Silva, C. B. (2019). Transovarial transmission and finding of Trypanosoma rhipicephalis in the hemolymph of Rhipicephalus sanguineus sensu lato. Brazilian Journal of Veterinary Medicine, 41(1), e106819. https://doi.org/10.29374/bjvm106819
Section
Scientific articles

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